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|Antigen||BCL2-Like 1 (BCL2L1) Antibodies|
|Conjugate||This BCL2L1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|4 references available|
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Product Details anti-BCL2L1 AntibodyTarget Details BCL2L1 Application Details Handling References for anti-BCL2L1 antibody (ABIN967340) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
|Purification||Purified from antiserum by negative adsorption and affinity chromatography.|
1) 51-9000093: Rabbit Anti-Serum-Bcl-X.
Quantity: 0.1 ml (1 ea).
Immunogen: Human Bcl-X aa. 46-66 synthetic peptide.
Isotype: Rabbit Ig.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide and Less or equal than 0.03% thimerosal
2) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
|Immunogen||Human Bcl-X synthetic peptide|
Target Details BCL2L1Product Details anti-BCL2L1 Antibody Application Details Handling References for anti-BCL2L1 antibody (ABIN967340) Images back to top
|Alternative Name||Bcl-x (BCL2L1 Antibody Abstract)|
|Background||Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. The first member of this multifamily, Bcl-2, was discovered in the mid 1980s through its involvement in t(14,18) chromosomal translocations commonly found in a subset of follicular B-cell lymphomas. Bcl-2 blocks apoptosis, and translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Deregulation of the Bcl-2 gene, by translocations or other mechanisms, appears to contribute to tumorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. A variety of Bcl-2 homologues have since been described in humans and other mammals, leading to the definition of a multigene family. Like Bcl-2, some Bcl-2 family members block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-X is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-X-L (long) is a 241 amino acid protein that is 47% homologous to Bcl-2 on the amino acid level. Bcl-X-S (short) is a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. bcl-X-L blocks cell death, whereas Bcl-X-S inhibits Bcl-2 and promotes cell death. The antibodies recognize both mouse and human Bcl-X-L (long) and Bcl-X-S (short) proteins. A synthetic peptide corresponding to amino acids 46-66 of human Bcl-X was used as immunogen. This region corresponds to a unique domain that lacks homolog with other known members of the Bcl-2 family.|
|Pathways||Apoptosis, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling References for anti-BCL2L1 antibody (ABIN967340) Images back to top
|Application Notes||Applications include western blot analysis (1:1000 - 1:2000)2-4 and immunohistochemical staining (1:500 - 1:2000) of frozen tissue sections and of formalinfixed or Bouin's-fixed, paraffin-embedded tissue sections. The antiserum should be stored at 4°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C).|
Related Products: ABIN967390, ABIN968535
|Restrictions||For Research Use only|
HandlingProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details References for anti-BCL2L1 antibody (ABIN967340) Images back to top
|Buffer||Aqueous buffered solution.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-BCL2L1 antibody (ABIN967340)Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling Images back to top
|Product cited in:||
Alexander-Miller, Derby, Sarin, Henkart, Berzofsky: "Supraoptimal peptide-major histocompatibility complex causes a decrease in bc1-2 levels and allows tumor necrosis factor alpha receptor II-mediated apoptosis of cytotoxic T lymphocytes." in: The Journal of experimental medicine, Vol. 188, Issue 8, pp. 1391-9, 1998
Reed, Miyashita, Krajewski, Takayama, Aime-Sempe, Kitada, Sato, Wang, Harigai, Hanada, Krajewska, Kochel, Millan, Kobayashi: "Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma." in: Cancer treatment and research, Vol. 84, pp. 31-72, 1996
Krajewski, Krajewska, Shabaik, Wang, Irie, Fong, Reed: "Immunohistochemical analysis of in vivo patterns of Bcl-X expression." in: Cancer research, Vol. 54, Issue 21, pp. 5501-7, 1994
Ohta, Iwai, Kasahara, Taniguchi, Krajewski, Reed, Miyawaki: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996
ImagesProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling References for anti-BCL2L1 antibody (ABIN967340) back to top