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anti-Pig (Porcine) BCL2L1 antibody for Immunocytochemistry

Recommended BCL2L1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 1 (BCL2L1) Antibodies
  • Bcl(X)L
  • Bcl-X
  • bcl-x
  • bcl-X
  • bcl-x(l)
  • Bcl-xl
  • bcl-xl
  • bcl-XL
  • bcl-xL
  • Bcl-XL
  • BCL-XL
  • Bcl-xL
  • BCL-XL/S
  • bcl-xl/s
  • bcl-xs
  • Bcl-Xs
  • Bcl-xS
  • bcl-xS
  • Bcl2l
  • bcl2l
  • BCL2L
  • BCL2L1
  • BclX
  • Bclx
  • BCLX
  • bclx
  • bclxl
  • BCLXL
  • BCLXS
  • PPP1R52
  • xR11
Epitope
N-Term, AA 30-70
152
34
27
22
19
14
13
13
11
11
11
7
7
7
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Cat (Feline), Human, Pig (Porcine), Sheep (Ovine)
479
304
277
137
16
8
6
5
4
3
2
2
2
1
Host
Rabbit
281
232
3
3
Clonality
Polyclonal
Conjugate
This BCL2L1 antibody is un-conjugated
23
15
10
9
7
7
6
5
5
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Western Blotting (WB)
402
190
165
156
145
126
124
95
91
23
7
4
3
3
2
1
1
Supplier
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Catalog No. ABIN259856
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN4283650 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283651 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283652 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283653 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see SPM165
0.0009965416 ABIN4283654 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see SPM165
0.0009965416 ABIN4283655 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see SPM165
0.0009965416 ABIN4283656 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see 2H12
0.0009965416 ABIN4283657 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see 2H12
0.0009965416 ABIN4283658 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 3-14 Log in to see 2H12
0.0009965416 ABIN4283661 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283662 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283663 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283664 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283665 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283666 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283667 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283668 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283669 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283671 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a AA 3-14 Log in to see BX006
0.0009965416 ABIN4283672 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a AA 3-14 Log in to see BX006

Similar anti-BCL2L1 Antibodies

Application / Reactivity Human Pig (Porcine) Dog (Canine) Cat (Feline) Sheep (Ovine)
Western Blotting (WB) 388 Antibodies 131 Antibodies 17 Antibodies 3 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Immunoprecipitation (IP) 128 Antibodies 71 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 182 Antibodies 101 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 158 Antibodies 13 Antibodies 6 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 91 Antibodies 72 Antibodies 4 Antibodies

General

Antigen BCL2-Like 1 (BCL2L1) Antibodies
Epitope N-Term, AA 30-70
(152), (34), (27), (22), (19), (14), (13), (13), (11), (11), (11), (7), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Cat (Feline), Human, Pig (Porcine), Sheep (Ovine)
(479), (304), (277), (137), (16), (8), (6), (5), (4), (3), (2), (2), (2), (1)
Host Rabbit
(281), (232), (3), (3)
Clonality
Polyclonal
Conjugate This BCL2L1 antibody is un-conjugated
(23), (15), (10), (9), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Western Blotting (WB)
(402), (190), (165), (156), (145), (126), (124), (95), (91), (23), (7), (4), (3), (3), (2), (1), (1)
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Product Details anti-BCL2L1 Antibody

Target Details BCL2L1 Application Details Handling Images
Specificity There are three known isoforms of Bcl-X, Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this has 100% identity to all three isoforms.
Purification affinity purified
Immunogen Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 30-70). Immunogen sequence has 93% homology to porcine, 87% to mouse and 81% to rat

Target Details BCL2L1

Product Details anti-BCL2L1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-XL (BCL2L1 Antibody Abstract)
Background Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x isexpressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in thecytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers withvarious proteins, including Bax, Bak and Bcl-2 (2). It has been found thatheterodimerization with Bax does not seem to be required for anti-apoptotic activity (3).Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strongpossibility that this property plays a role in heterodimerization-independent cell survival(4). The Bcl-X(S) isoform promotes apoptosis. Alternate Names: anti-Apoptosis regulator Bcl X antibody, anti-Bcl 2 like 1 antibody, anti-Bcl 2 like 1protein antibody, anti-Bcl xL antibody, anti-BCL XL/S antibody, anti-Bcl xS antibody,anti-Bcl2 like1 antibody, anti-BCL2L antibody, anti-BCL2L1 antibody, anti-Bclx antibody,anti-DKFZp781P2092 antibody.
Gene Symbol: BCL2L1
Gene ID 598
UniProt Q07817
Research Area Cancer
Pathways Apoptosis

Application Details

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling Images back to top
Application Notes This BCL2L1 antibody is useful for Western blot, where a band is seen ~26 kDa. This antibody is not useful for ICC/IF.
Protocol Protocol specific for BCL2L1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Bcl-X primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2L1 antibody (BCL2-Like 1) (N-Term) (ABIN259856) Detection of Bcl-x in A431 whole cell extracts using ABIN259855.