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anti-Human FASL antibody for Cell-ELISA

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Epitope
Extracellular Domain
29
25
14
12
11
9
7
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
274
138
65
3
3
1
1
1
1
1
Host
Mouse
146
126
61
29
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
196
162
87
63
57
50
30
29
17
12
11
11
11
6
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN108724
$ 358.05
Plus shipping costs $45.00

Top referenced anti-FASL antibody for Cell-ELISA

Similar anti-FASL Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 82 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 120 Antibodies
Functional Studies (Func) 23 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 66 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope Extracellular Domain
(29), (25), (14), (12), (11), (9), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(274), (138), (65), (3), (3), (1), (1), (1), (1), (1)
Host Mouse
(146), (126), (61), (29), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS)
(196), (162), (87), (63), (57), (50), (30), (29), (17), (12), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN108724) Images
Specificity GM5F4 recognises FASL transiently expressed on the cell surface of transfected BOSC cells.
Purification Protein G
Immunogen genetic immunisation with cDNA encoding human FasL (extracellular domain)
Clone GM-5F4
Isotype IgG1

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN108724) Images back to top
Antigen
Alternative Name FASL (FASL Antibody Abstract)
Background The FAS ligand (FASL, CD95), a member of the tumor necrosis factor family, induces apoptosis in FAS-bearing cells. GM5F4 against this transmembrane receptor was generated by genetic immunisation.
UniProt P48023
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN108724) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD95, Fas ligand

Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN108724) Images back to top
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-FASL Antibody (ABIN108724)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Suda, Hashimoto, Tanaka, Ochi, Nagata: "Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2045-50, 1998

Nagata: "Apoptosis mediated by the Fas system." in: Progress in molecular and subcellular biology, Vol. 16, pp. 87-103, 1997

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN108724) back to top
Supplier Images
 image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (Extracellular Domain) (ABIN108724) Specificity testing of GM5F4. BOSC cells were transiently transfected with an express...