anti-Human FASL antibody for Cell-ELISA

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Epitope
Extracellular Domain
26
19
17
16
12
9
8
7
7
6
5
5
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
421
174
89
36
7
5
5
3
3
2
1
1
Host
Mouse
258
176
49
32
29
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
45
31
27
12
12
9
9
9
9
8
8
8
8
8
7
6
6
4
3
2
2
2
2
2
1
1
1
1
1
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
313
258
125
97
80
75
51
47
28
28
13
12
10
10
7
5
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN108724
$ 386.93
Plus shipping costs $45.00

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope Extracellular Domain
(26), (19), (17), (16), (12), (9), (8), (7), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(421), (174), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Host Mouse
(258), (176), (49), (32), (29), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(45), (31), (27), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS)
(313), (258), (125), (97), (80), (75), (51), (47), (28), (28), (13), (12), (10), (10), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN108724) Images
Specificity GM5F4 recognises FASL transiently expressed on the cell surface of transfected BOSC cells.
Purification Protein G
Immunogen genetic immunisation with cDNA encoding human FasL (extracellular domain)
Clone GM-5F4
Isotype IgG1

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN108724) Images back to top
Antigen
Alternative Name FASL (FASL Antibody Abstract)
Background The FAS ligand (FASL, CD95), a member of the tumor necrosis factor family, induces apoptosis in FAS-bearing cells. GM5F4 against this transmembrane receptor was generated by genetic immunisation.
UniProt P48023
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN108724) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD95, Fas ligand

Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN108724) Images back to top
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-FASL antibody (ABIN108724)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Suda, Hashimoto, Tanaka, Ochi, Nagata: "Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2045-50, 1998

Nagata: "Apoptosis mediated by the Fas system." in: Progress in molecular and subcellular biology, Vol. 16, pp. 87-103, 1997

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN108724) back to top
Supplier Images
 image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (Extracellular Domain) antibody (ABIN108724) Specificity testing of GM5F4. BOSC cells were transiently transfected with an express...