anti-Human FASL antibody for Functional Studies

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Reactivity
Human
421
174
89
36
7
5
5
3
3
2
1
1
Host
Mouse
258
176
49
32
29
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
45
31
27
12
12
9
9
9
9
8
8
8
8
8
7
6
6
4
3
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
313
258
125
97
80
74
51
47
28
27
13
12
10
9
7
5
3
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN120316
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1105943 Func FACS Mouse IgG1 Log in to see B-R17
1 ABIN187308 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see 5G51 1
1 ABIN1981904 Func ICC FACS IP WB PE Mouse IgG1 Log in to see NOK-1 5
1 ABIN2749018 Func ICC FACS IP WB Mouse IgG1 Log in to see NOK-1 5
1 ABIN187307 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see 2C101
1 ABIN2749049 Func ICC FACS IP WB Mouse IgG1 Log in to see NOK-1 5
1 ABIN199151 Func FACS Neut Mouse IgG1 Extracellular Domain Log in to see
1 ABIN199147 Func ICC FACS Neut Mouse IgG1 Extracellular Domain Log in to see
1 ABIN2478850 Func FACS IP ELISA Mouse IgG1 Log in to see 10F2 3
1 ABIN1722561 Func FACS ELISA Mouse IgG1 Log in to see B-R17
1 ABIN3199538 FACS Func ICC IP WB Mouse IgG1 Log in to see NOK-1
1 ABIN5540784 FACS Func ICC IF IP WB Mouse IgG1 Log in to see NOK-1
1 ABIN3199540 FACS Func ICC IP WB PE Mouse IgG1 Log in to see NOK-1
1 ABIN3199539 FACS Func ICC IP WB Mouse IgG1 Log in to see NOK-1
1 ABIN5540783 Func Mouse IgG1 Log in to see NOK-1
1 ABIN2567283 Func FACS IP WB Mouse IgG1 kappa Log in to see NOK-1
1 ABIN2567284 Func FACS Mouse IgG1 kappa Log in to see NOK-1
1 ABIN217751 Func FACS IP ELISA WB Mouse IgG1 Log in to see
1 ABIN639317 Func FACS IP ELISA Mouse Log in to see

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human
(421), (174), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Host Mouse
(258), (176), (49), (32), (29), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(45), (31), (27), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(313), (258), (125), (97), (80), (74), (51), (47), (28), (27), (13), (12), (10), (9), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN120316) Images
Specificity This antibody recognises the CD178 cell surface antigen, a 40 kDa glycoprotein also known as Fas ligand (CD95L). Clone 10F2 is reported to block the activity of Fas ligand.
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Clone 1OF2
Isotype IgG1

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN120316) Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gene ID 356
NCBI Accession NP_000630
UniProt P48023
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN120316) Images back to top
Application Notes Flow Cytometry: 1/25 - 1/50, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. ELISA: This antibody may be used in combination with clone 14C2 (ABIN120319) in ELISA assays. Immunoprecipitation. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN120316) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-FASL antibody (ABIN120316)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Legembre, Moreau, Daburon, Moreau, Taupin: "Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas." in: Cell death and differentiation, Vol. 9, Issue 3, pp. 329-39, 2002

Ferry-Dumazet, Garnier, Mamani-Matsuda, Vercauteren, Belloc, Billiard, Dupouy, Thiolat, Kolb, Marit, Reiffers, Mossalayi: "Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells." in: Carcinogenesis, Vol. 23, Issue 8, pp. 1327-33, 2002

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN120316) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN120316) Staining of CD178 transfected CHO cells with MOUSE ANTI HUMAN CD178 (ABIN120316)