anti-Human FASL antibody for Neutralization

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Reactivity
Human
421
174
89
36
7
5
5
3
3
2
1
1
Host
Mouse
258
176
49
32
29
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
45
31
27
12
12
9
9
9
9
8
8
8
8
8
7
6
6
4
3
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
313
257
124
97
80
74
51
47
28
28
13
11
10
10
7
5
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1176953
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177002 FACS IP Neut WB Mouse IgG1 Log in to see NOK-1 9
1 ABIN465173 IHC (p) Neut ELISA WB Goat Log in to see Polyclonal
1 ABIN2689020 FACS IP Neut ELISA WB Mouse IgG2a Log in to see NOK 3
1 ABIN199148 FACS IHC (fro) IHC (p) Neut Mouse IgG1 Extracellular Domain Log in to see
1 ABIN199151 Func FACS Neut Mouse IgG1 Extracellular Domain Log in to see
1 ABIN199147 Func ICC FACS Neut Mouse IgG1 Extracellular Domain Log in to see
1 ABIN1817978 FACS Neut Biotin Hamster IgG Log in to see FLIM58
1 ABIN2264875 FACS Neut IHC Mouse IgG1 Log in to see 6D386
1 ABIN400619 IHC Neut ELISA WB Goat IgG Log in to see Polyclonal

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human
(421), (174), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Host Mouse
(258), (176), (49), (32), (29), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(45), (31), (27), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
(313), (257), (124), (97), (80), (74), (51), (47), (28), (28), (13), (11), (10), (10), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN1176953) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL
Clone NOK-2
Isotype IgG2a

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN1176953) Images back to top
Antigen
Alternative Name CD178 (FASL Antibody Abstract)
Background Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung. FasL is a 40kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. NOK-2 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al.
Synonyms: Fas Ligand, CD95 Ligand
Molecular Weight 40, 26 kDa
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN1176953) Images back to top
Application Notes IP: Applications include immunoprecipitation (1-2 µg/one million cells).
Functional Assay: NOK-2 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediating killing. The NA/LE format of NOK-2 (Cat. No. 556375) should be used for all functional assays. NOK-2 and a related human FasL clone, NOK-1 [Cat. No. 556371 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes.
Flow Cytometry: Another clone, NOK-1, (556372, 556373) are recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1.
Western Blot: Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 (Cat. No. 556387) is suggested for the western blot analysis.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN1176953) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-FASL antibody (ABIN1176953)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Product cited in:

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN1176953) back to top
Supplier Images
Western Blotting (WB) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN1176953) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody