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|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
|3 references available|
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Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling References for anti-FASL antibody (ABIN1176953) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN1176953) Images back to top
|Alternative Name||CD178 (FASL Antibody Abstract)|
Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung. FasL is a 40kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. NOK-2 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al.
Synonyms: Fas Ligand, CD95 Ligand
|Molecular Weight||40, 26 kDa|
|Pathways||Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN1176953) Images back to top
IP: Applications include immunoprecipitation (1-2 µg/one million cells).
Functional Assay: NOK-2 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediating killing. The NA/LE format of NOK-2 (Cat. No. 556375) should be used for all functional assays. NOK-2 and a related human FasL clone, NOK-1 [Cat. No. 556371 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes.
Flow Cytometry: Another clone, NOK-1, (556372, 556373) are recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1.
Western Blot: Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 (Cat. No. 556387) is suggested for the western blot analysis.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN1176953) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-FASL antibody (ABIN1176953)Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
|Product cited in:||
Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996
Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995
Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN1176953) back to top