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|Antigen||Lamin B1 (LMNB1) ELISA Kits|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||0.15-10 ng/mL|
|Minimum Detection Limit||0.15 ng/mL|
|Supplier||Log in to see|
Product Details Lamin B1 ELISA KitTarget details Application Details Handling Images
|Purpose||ELISA Kit for Lamin B1 (LMNB1)|
|Sample Type||Cell Lysate, Tissue Homogenate, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Lamin B1 (LMNB1).
Target detailsProduct Details Lamin B1 ELISA Kit Application Details Handling Images back to top
|Alternative Name||Lamin B1 (LMNB1 ELISA Kit Abstract)|
|Research Area||Signaling, Cytoskeleton, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details Lamin B1 ELISA Kit Target details Handling Images back to top
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B1 (LMNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B1 (LMNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B1 (LMNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin B1 (LMNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin B1 (LMNB1) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
HandlingProduct Details Lamin B1 ELISA Kit Target details Application Details Images back to top
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.