TMB High Sensitivity Substrate Solution

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Application
ELISA
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Characteristics The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.
Components TMB High Sensitivity Substrate Solution
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Storage 4 °C
Storage Comment The TMB High Sensitivity Substrate Solution should be stored between 2°C and 8°C.
Background publications Nogales, Baker, Ortiz-Riaño, Dewhurst, Topham, Martínez-Sobrido: "Influenza A virus attenuation by codon deoptimization of the NS gene for vaccine development." in: Journal of virology, Vol. 88, Issue 18, pp. 10525-40, 2014 (PubMed).

Querol, Nogales-Gadea, Rojas-Garcia, Diaz-Manera, Pardo, Ortega-Moreno, Sedano, Gallardo, Berciano, Blesa, Dalmau, Illa: "Neurofascin IgG4 antibodies in CIDP associate with disabling tremor and poor response to IVIg." in: Neurology, Vol. 82, Issue 10, pp. 879-86, 2014 (PubMed).

Fan, Monk, Hou, Callway, Vincent, Weeks, Yang, Chapkin: "Characterization of an arachidonic acid-deficient (Fads1 knockout) mouse model." in: Journal of lipid research, Vol. 53, Issue 7, pp. 1287-95, 2012 (PubMed).

Eisenach, Roghi, Fogarasi, Murphy, English: "MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src." in: Journal of cell science, Vol. 123, Issue Pt 23, pp. 4182-93, 2010 (PubMed).

Zeng, Wang, Cao, An, Zeng, Han, Song, Deng: "Immunopotentiation of Caffeoyl Glycoside from Picrorhiza scrophulariiflora on activation and cytokines secretion of immunocyte in vitro." in: International immunopharmacology, Vol. 8, Issue 12, pp. 1707-12, 2008 (PubMed).