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Adenosine A2a Receptor antibody (3rd Intracellular Domain)

Name: Adenosine A2a Receptor antibody (3rd Intracellular Domain)
SKU: ABIN305729
Price: 715 USD
Availability: InStock

ADORA2A Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig WB, IHC, FACS, IHC (p), IHC (pfa) Host: Mouse Monoclonal 7F6-G5-A2-7F6 unconjugated

Anti-ADORA2A antibody IHC of human brain, putamen. (Adenosine A2a Receptor antibody (3rd Intracellular Domain))

IHC

Human Brain, Putamen (formalin-fixed, paraffin-embedded) stained with ADORA2A antibody ABIN305729 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen. (Adenosine A2a Receptor antibody (3rd Intracellular Domain))

IHC (p)

2 images

Catalog No. ABIN305729

Datasheet as PDF

Target See all Adenosine A2a Receptor (ADORA2A) Antibodies

Adenosine A2a Receptor (ADORA2A)
Binding Specificity
12

9

7

7

7

7

5

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
3rd Intracellular Domain
Reactivity
79

29

28

8

4

3

2

2

2

2

1

1
Human, Mouse, Rat, Dog, Rabbit, Guinea Pig
Host
70

10

2
Mouse
Compatible Secondaries
Clonality
74

7

1
Monoclonal
Conjugate
51

7

4

4

3

2

1

1

1

1

1

1

1

1

1

1

1
This Adenosine A2a Receptor antibody is un-conjugated
Application
69

35

29

17

13

11

8

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Brand

IHC-plus™

Specificity

Recognizes human adenosine receptor A2A at ~43kD.

Purification

Protein G purified

Immunogen

6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.

Type of Immunogen: Recombinant protein

Clone

7F6-G5-A2-7F6

Isotype

IgG2a

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ADORA2A Reactivity: Human WB, ELISA, IHC, FACS, ICC, Neut Host: Mouse Monoclonal 6A6G11 unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (C-Term) antibody
ADORA2A Reactivity: Human WB Host: Goat Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 100-200) antibody
ADORA2A Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 1-412) antibody
ADORA2A Reactivity: Human WB, FACS Host: Mouse Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) antibody
ADORA2A Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 291-412) antibody
ADORA2A Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 165-300) antibody
ADORA2A Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (Internal Region) antibody
ADORA2A Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 347-381), (C-Term) antibody
ADORA2A Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB52103 unconjugated

anti-Adenosine A2a Receptor (ADORA2A) (AA 1-412) antibody
ADORA2A Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo, IHC, IHC-P (5 μg/mL), IHC-Pfa (1 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.01M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

Adenosine A2a Receptor (ADORA2A)

Alternative Name

ADORA2A/Adenosine A2A Receptor (ADORA2A Products)

Synonyms

ADORA2A antibody, adora2a.1 antibody, zgc:194773 antibody, A2aR antibody, ADORA2 antibody, RDC8 antibody, A2AAR antibody, AA2AR antibody, A2ar antibody, ADENO antibody, Adora2l1 antibody, adora2a.2 antibody, zgc:193802 antibody, zgc:193822 antibody, adenosine A2a receptor antibody, adenosine A2a receptor a antibody, adenosine A2a receptor L homeolog antibody, adenosine A2a receptor b antibody, ADORA2A antibody, Adora2a antibody, adora2aa antibody, adora2a.L antibody, adora2a antibody, adora2ab antibody

Background

Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gene ID

135

UniProt

P29274

Pathways

Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints