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CS antibody

Name: CS antibody
Brand: antibodies-online
SKU: ABIN2856769
Price: 609.18 USD
Availability: InStock

KO Validated CS Reactivity: Human WB, IP, IF, ICC, IHC (p), IHC (wm) Host: Rabbit Polyclonal unconjugated

IHC-P Image Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis.

IHC

WB Image Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis.

ICC/IF Image Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.

ICC/IF Image Immunofluorescence analysis of methanol-fixed A431, using Citrate synthetase, antibody at 1:200 dilution.

IP Image Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3], Western blot analysis was performed using Citrate synthetase antibody [N2C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Citrate synthetase, antibody at 1:500 dilution.

IHC

IHC-P Image Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis.

IHC

Respiratory capacity is impaired in BAT from LRPPRC fat-specific knockout (FKO) mice.

Influence of SIRT3 depletion on protein expression of mitochondrial content markers.

Impaired respiratory capacity attenuates thermogenic and oxidative gene expression in BAT from LRPPRC FKO mice living at 30 °C.

13 images

(5 references)

Catalog No. ABIN2856769

Datasheet as PDF

Target See all CS Antibodies

CS (Citrate Synthase (CS))
Reactivity
40

13

12

10

1
Human
Host
38

14

1
Rabbit
Compatible Secondaries
Clonality
38

15
Polyclonal
Conjugate
23

8

4

4

2

2

2

2

2

2

1

1
This CS antibody is un-conjugated
Application
37

25

19

11

10

8

7

6

2

2

2

2

2

2

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity

Chicken, Frog, Human, Monkey, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics

Rabbit Polyclonal antibody to Citrate synthetase (citrate synthase)
Citrate synthetase antibody [N2C3]

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Isotype

IgG
anti-Citrate Synthase (CS) (AA 103-394) antibody
CS Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Citrate Synthase (CS) (AA 174-428) antibody
CS Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Citrate Synthase (CS) (AA 56-312) antibody
CS Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Citrate Synthase (CS) antibody
CS Reactivity: Human WB, IF, IHC (p) Host: Mouse Monoclonal CL2545 unconjugated

anti-Citrate Synthase (CS) antibody
CS Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal CL2561 unconjugated

anti-Citrate Synthase (CS) antibody (FITC)
CS Reactivity: Human WB, IHC, ELISA, IHC (p) Host: Rabbit Polyclonal FITC

anti-Citrate Synthase (CS) antibody (Biotin)
CS Reactivity: Human WB, IHC, ELISA, IHC (p) Host: Rabbit Polyclonal Biotin

anti-Citrate Synthase (CS) antibody (HRP)
CS Reactivity: Human WB, IHC, ELISA, IHC (p) Host: Rabbit Polyclonal HRP

anti-Citrate Synthase (CS) (Internal Region) antibody
CS Reactivity: Human, Mouse, Chicken WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Citrate Synthase (CS) antibody
CS Reactivity: Human, Mouse, Rat WB, IF, IC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: mouse brain , rat brain , Rat muscle , 293T
Validation: KO/KD

Restrictions

For Research Use only
Format

Liquid

Concentration

0.65 mg/mL

Buffer

1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Ding, Rose, Sun, Wu, Chen, Lin, Yang, Chen, Lee, Xu, Tian, Akinwuntan, Zhao, Guan, Zhou, Chi: "MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis." in: Nature metabolism, Vol. 2, Issue 3, pp. 270-277, (2020) (PubMed).

Leandro, Violante, Argmann, Hagen, Dodatko, Bender, Zhang, Williams, Bachmann, Auwerx, Yu, Houten: "Mild inborn errors of metabolism in commonly used inbred mouse strains." in: Molecular genetics and metabolism, Vol. 126, Issue 4, pp. 388-396, (2019) (PubMed).

Kim, Men, Liang, Receno, Brutsaert, Korol, Heffernan, DeRuisseau: "Effects of long-term exposures to low iron and branched-chain amino acid containing diets on aging skeletal muscle of Fisher 344 × Brown Norway rats." in: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme, Vol. 43, Issue 2, pp. 165-173, (2018) (PubMed).

Nam, Akie, Sanosaka, Craige, Kant, Keaney, Cooper: "Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression." in: Scientific reports, Vol. 7, Issue 1, pp. 2013, (2018) (PubMed).

Chang, Ulrich, Suliman, Piantadosi: "Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis." in: Free radical biology & medicine, Vol. 78, pp. 179-89, (2015) (PubMed).
Target

CS (Citrate Synthase (CS))

Alternative Name

citrate synthase (CS Products)

Synonyms

2610511A05Rik antibody, 9030605P22Rik antibody, BB234005 antibody, Cis antibody, ahl4 antibody, wu:fb58e04 antibody, zgc:55507 antibody, citrate synthase antibody, citrate synthase L homeolog antibody, Cs antibody, CS antibody, cs.L antibody, cs antibody

Background

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

Cellular Localization: Mitochondrion matrix

Molecular Weight

52 kDa

Gene ID

1431

UniProt

O75390