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ENO1 antibody (AA 1-434)

Name: ENO1 antibody (AA 1-434)
SKU: ABIN560741
Price: 534.67 USD
Availability: InStock

ENO1 Reactivity: Human WB, ELISA, IF, IP, IHC (p) Host: Mouse Monoclonal 8G8 unconjugated

ENO1 monoclonal antibody (M01), clone 8G8. (ENO1 antibody (AA 1-434))

Detection limit for recombinant GST tagged ENO1 is approximately 0. (ENO1 antibody (AA 1-434))

ELISA

Immunoprecipitation of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with ENO1 MaxPab rabbit polyclonal antibody. (ENO1 antibody (AA 1-434))

Western Blot detection against Immunogen (73. (ENO1 antibody (AA 1-434))

Immunoperoxidase of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. (ENO1 antibody (AA 1-434))

ISt

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell. (ENO1 antibody (AA 1-434))

ENO1 monoclonal antibody (M01), clone 8G8 Western Blot analysis of ENO1 expression in MCF-7 . (ENO1 antibody (AA 1-434))

Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8. (ENO1 antibody (AA 1-434))

8 images

(6 references)

Catalog No. ABIN560741

Datasheet as PDF

Target See all ENO1 Antibodies

ENO1 (Enolase 1 (ENO1))
Binding Specificity
14

7

6

6

5

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-434
Reactivity
84

50

31

15

11

6

6

6

4

4

3

3

3

2

1

1

1

1
Human
Host
84

14

3
Mouse
Compatible Secondaries
Clonality
77

23
Monoclonal
Conjugate
55

12

8

5

3

3

3

3

3

3

1

1

1
This ENO1 antibody is un-conjugated
Application
68

34

29

25

19

12

10

9

5

4

2

2

2

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Mouse monoclonal antibody raised against a full length recombinant ENO1.

Sequence

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

8G8

Isotype

IgG1
anti-Enolase 1 (ENO1) (AA 2-434) antibody
ENO1 Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 8H9G12 unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Enolase 1 (ENO1) antibody
ENO1 Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (Middle Region) antibody
ENO1 Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Dog, Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 405-434), (C-Term) antibody
ENO1 Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB19995 unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 8G3 unconjugated

anti-Enolase 1 (ENO1) antibody
KO Validated ENO1 Reactivity: Human WB, IF, ICC, IHC (p), DB, IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 246-295) antibody
ENO1 Reactivity: Human, Horse, Pig, Monkey, Chicken WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 2-428) antibody
ENO1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Dinh, Kuhl, Görtz, Goldbrunner, Timmer: "Expression of ENO1 Is Up-regulated in Low-grade Glioma and Positively Correlated With Meningioma Grade." in: Anticancer research, Vol. 42, Issue 5, pp. 2319-2326, (2022) (PubMed).

Zakrzewicz, Didiasova, Zakrzewicz, Hocke, Uhle, Markart, Preissner, Wygrecka: "The interaction of enolase-1 with caveolae-associated proteins regulates its subcellular localization." in: The Biochemical journal, Vol. 460, Issue 2, pp. 295-307, (2014) (PubMed).

Yu, Shen, Yu, Zhong, Zhang, Zhang, Zhao, Li, Chen, Liu, Yang: "Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis." in: Molecular bioSystems, Vol. 7, Issue 6, pp. 1908-16, (2011) (PubMed).

Chu, Hsu, Liao, Shih, Hou, Liu: "Overexpression of ?-enolase correlates with poor survival in canine mammary carcinoma." in: BMC veterinary research, Vol. 7, pp. 62, (2011) (PubMed).

Ho, Chang, Shih, Wu, Chang, Chen, Chou: "Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor." in: Analytical chemistry, Vol. 82, Issue 14, pp. 5944-50, (2010) (PubMed).

Zhao, Chang, Li, Shedden, Thomas, Misek, Manoharan, Giordano, Beer, Lubman: "Comparative proteomics analysis of Barrett metaplasia and esophageal adenocarcinoma using two-dimensional liquid mass mapping." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 987-99, (2007) (PubMed).
Target

ENO1 (Enolase 1 (ENO1))

Alternative Name

ENO1 (ENO1 Products)

Synonyms

enolase 1 antibody, F2P9.10 antibody, F2P9_10 antibody, Pgh1 antibody, pENO1 antibody, enolase 1 antibody, phosphopyruvate hydratase antibody, ENO1 antibody, eno1 antibody

Background

Full Gene Name: enolase 1, (alpha)
Synonyms: ENO1L1,MBP-1,MPB1,NNE,PPH

Gene ID

2023