SEMA4B Protein (AA 31-703) (His tag)
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- Target See all SEMA4B Proteins
- SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-703
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SEMA4B protein is labelled with His tag.
- Purpose
- Mouse SEMA4B Protein
- Sequence
- Leu31-Glu703
- Characteristics
- Recombinant Mouse SEMA4B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu31-Glu703.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))
- Alternative Name
- SEMA4B (SEMA4B Products)
- Synonyms
- sema4b Protein, MGC80974 Protein, SEMA4B Protein, SEMAC Protein, SemC Protein, Semac Protein, mKIAA1745 Protein, semaphorin 4B Protein, semaphorin 4B S homeolog Protein, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B Protein, SEMA4B Protein, sema4b.S Protein, Sema4b Protein
- Background
- Semaphorin 4B (SEMA4B) inhibits the invasion of non-small cell lung cancer (NSCLC) through PI3K-dependent suppression of MMP9 activation. SEMA4B may induce FoxO1 nuclear retention through suppressing PI3K/Akt signaling pathway, which subsequently inhibited cell growth through the direct nuclear target of FoxO1, p21. A role of SEMA4B in suppressing NSCLC growth, besides its role in inhibiting cell metastasis, and highlights SEMA4B as a promising therapeutic target for NSCLC.
- Molecular Weight
- 75.7 kDa. Due to glycosylation, the protein migrates to 78-83 kDa based on Tris-Bis PAGE result.
- UniProt
- Q62179
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