Splicing Factor 1 antibody (AA 160-266)
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- Target See all Splicing Factor 1 (SF1) Antibodies
- Splicing Factor 1 (SF1)
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Binding Specificity
- AA 160-266
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Splicing Factor 1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Brand
- Picoband™
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for splicing factor 1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- Immunogen
- E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
- Top Product
- Discover our top product SF1 Primary Antibody
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anti-Splicing Factor 1 (SF1) (AA 1-110) antibody
SF1 Reactivity: Human WB, ELISA, IF, IHC (p), IP Host: Mouse Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 1-111) antibodySF1 Reactivity: Human WB, ELISA, IHC, IF, IHC (p) Host: Mouse Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 517-566) antibodySF1 Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Monkey, Pig, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibodySF1 Reactivity: Human, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 Reactivity: Human, Mouse WB, ELISA, IHC, IF/ICC Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio) WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (pSer82) antibodySF1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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- Target
- Splicing Factor 1 (SF1)
- Alternative Name
- SF1 (SF1 Products)
- Synonyms
- BBP antibody, D11S636 antibody, MBBP antibody, ZCCHC25 antibody, ZFM1 antibody, ZNF162 antibody, BB094781 antibody, CW17R antibody, MZFM antibody, WBP4 antibody, Zfp162 antibody, CG5836 antibody, Dmel\\CG5836 antibody, cg5836 antibody, dSF1 antibody, p70 antibody, SF1 antibody, sf1 antibody, wu:fc09f06 antibody, znf162 antibody, MWD22.25 antibody, MWD22_25 antibody, SF-1 antibody, splicing factor 1 antibody, Splicing factor 1 antibody, splicing factor-like protein antibody, nuclear receptor subfamily 5 group A member 1 antibody, splicing factor 1 S homeolog antibody, SF1 antibody, Sf1 antibody, sf1 antibody, AT5G51300 antibody, NR5A1 antibody, sf1.S antibody
- Background
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Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162
Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
- UniProt
- Q15637
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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