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CD200 antibody (Internal Region)

Name: CD200 antibody (Internal Region)
Brand: antibodies-online
SKU: ABIN3187966
Price: 391.95 USD
Availability: InStock
Rating: 5 (1 reviews)

CD200 Reactivity: Human WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966) (CD200 antibody (Internal Region))

IHC

Western Blotting (WB) image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966) (CD200 antibody (Internal Region))

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100. (CD200 antibody (Internal Region))

IHC

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100. (CD200 antibody (Internal Region))

IHC

6 images

(1 reference)

(1 validation)

Catalog No. ABIN3187966

Datasheet as PDF

Target See all CD200 Antibodies

CD200
Binding Specificity
15

10

9

8

6

3

2

1

1

1

1

1

1

1
Internal Region
Reactivity
88

23

21

2

2

2

2

2

1

1
Human
Host
56

45

13
Rabbit
Compatible Secondaries
Clonality
58

53
Polyclonal
Conjugate
38

14

12

10

8

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD200 antibody is un-conjugated
Application
53

52

31

25

16

14

13

8

7

5

4

3

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

CD200 Polyclonal Antibody detects endogenous levels of CD200 protein.

Characteristics

Rabbit Polyclonal to CD200.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

Synthesized peptide derived from the Internal region of human CD200 (aa 171-220)

Isotype

IgG
anti-CD200 (CD200) (AA 41-140) antibody
CD200 Reactivity: Human WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD200 (CD200) (AA 31-232) antibody
CD200 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD200 (CD200) antibody (PE)
CD200 Reactivity: Human FACS Host: Mouse Monoclonal OX-104 PE

anti-CD200 (CD200) antibody (FITC)
CD200 Reactivity: Human FACS Host: Mouse Monoclonal OX-104 FITC

anti-CD200 (CD200) antibody
CD200 Reactivity: Human FACS, IHC (fro), IHC (p) Host: Mouse Monoclonal OX-104 unconjugated

anti-CD200 (CD200) (AA 56-257) antibody
CD200 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6E8B11 unconjugated

anti-CD200 (CD200) antibody (APC)
CD200 Reactivity: Human FACS Host: Mouse Monoclonal OX-104 APC

anti-CD200 (CD200) (AA 31-232) antibody
CD200 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM156 unconjugated

anti-CD200 (CD200) antibody
CD200 Reactivity: Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

anti-CD200 (CD200) antibody (PerCP-Cy5.5)
CD200 Reactivity: Human FACS Host: Mouse Monoclonal OX-104 PerCP-Cy5.5

Application Notes

WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000,

Restrictions

For Research Use only
Validation #103457 (Immunohistochemistry)

by

David A. Clark, Department of Medicine and Department of Pathology and Molecular Medicine, McMaster University

No.

#103457

Date

11/30/2018

Antigen

CD200

Lot Number

6839401

Method validated

Immunohistochemistry

Positive Control

Human 7 week elective termination placenta

Negative Control

KLH antibody ABIN401183

Diluent

Notes

Passed. The CD200 antibody ABIN318966 specifically recognizes its antigen in human placental tissue.

Validation Images

Staining with ABIN318966 at dilutions 1:200, 1:150, 1:200, and 1:250 (two upper rows) using a 7 week elective termination of a missed abortion pregnancy. The upper panels show placental villus and associated decidual tissue, lower panels show staining of villi not associated with decidua. For comparison, tissue samples were stained with ABIN761396, anti-KLH antibody ABIN401183, and diluent alone without any antibody. Images were taken at 400x magnification. VC: placental villus core; T: trophoblast; CT: cytotrophoblast inner layer; ST: syncytiotrophoblast outer layer; DE: decidual epithelium; D: decidua.
Full Methods
Primary Antibody

ABIN3187966

Secondary Antibody

Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)

Full Protocol

Fix human placental tissue in 10% buffered formalin for 24h at RT (22-23°C), process, and embedded in paraffin.
Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
Affix sections to positively charged slides and air dry ON at RT.
Dewax the slides and hydrate on the automated Leica BOND Rx stainer.
Antigen retrieval on a Leica BOND RX automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
Stain slides with primary
rabbit anti- CD200 antibody (AA 45-95) (Antibodies-online, ABIN761396, lot 980502W),
rabbit anti-CD200 antibody (AA 170-220) (Antibodies-online, ABIN318966 lot 6839401) (AA 170-220), or
rabbit anti-KLH antibody (Antibodies-online, ABIN401183, lot 304770)
diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
Mount slides mounted in Fisher Chemical Permount Mounting Medium (Fisher Scientific, SP15-500, lot 162767).
Once the slide-coverslip edges are dry, scan the slides using Leica Imagescope (40x) and photograph into jpg files at 400x.

Experimental Notes

Several dilutions of ABIN318966 were tested and optimal staining was obtained at a dilution as low as 1:200.
The staining pattern of ABIN318966 differs from our result using ABIN761396 which matched RB846 and observations made by others. The result may be explainable by ABIN318966 reacting with variants of CD200 which lack upstream exon 3 coded AA that are eliminated along with exon 2 coded AA by alternative splicing so as to produce the truncated form of CD200 (CD200tr). Removing exon 2 + some exon 3 coded AA may expose epitopes in the downstream exon 3 + exon 4 region of CD200tr that are detected by antibody to the 170-220 AA region of CD200 that are in exon 4 of CD200 variant 1.
ABIN761396 stained villus trophoblasts as described in Clark et al. (2017).
ABIN761396 stained villus cytotrophoblasts and syncytiotrophoblasts but ABIN3187966 did not show convincing staining.
Format

Liquid

Concentration

1 mg/mL

Buffer

Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C.
Clark, Reihani, Arredondo, Ask, Foster: "CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells." in: Journal of reproductive immunology, Vol. 150, pp. 103477, (2022) (PubMed).
Target

CD200

Alternative Name

CD200 (CD200 Products)

Synonyms

MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody

Molecular Weight

31 kDa

Gene ID

4345

UniProt

P41217