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Sclerostin antibody (N-Term)

Name: Sclerostin antibody (N-Term)
SKU: ABIN390193
Price: 429 USD
Availability: InStock

SOST Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal RB12388 unconjugated

Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term) (ABIN390193 and ABIN2840682).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using A performed on the Leica® BOND RXm.

IHC (p)

All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: human liver lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 μg per lane.

All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilution Lane 1: human kidney lysates Lane 2: human liver lysates Lysates/proteins at 20 μg per lane.

Anti-SOST Antibody (N-term) at 1:2000 dilution + Recombinant human SOST protein lysate Lysates/proteins at 20 ng per lane.

5 images

(2 references)

Catalog No. ABIN390193

Datasheet as PDF

Target See all Sclerostin (SOST) Antibodies

Sclerostin (SOST)
Binding Specificity
15

10

8

7

3

3

2

2

1

1

1

1

1

1

1
AA 12-42, N-Term
Reactivity
64

51

34

16

15

15

2

1

1

1

1
Human, Mouse, Rat
Host
66

3

2

1
Rabbit
Compatible Secondaries
Clonality
67

5
Polyclonal
Conjugate
29

6

6

5

3

3

2

2

2

2

2

2

2

2

2

1

1
This Sclerostin antibody is un-conjugated
Application
69

30

24

23

13

11

5

4

3

2

2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

B

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.

Clone

RB12388

Isotype

IgG
anti-Sclerostin (SOST) (AA 25-213) antibody
SOST Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (AA 24-213) antibody
SOST Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (AA 24-213) antibody
SOST Reactivity: Human IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (N-Term) antibody
SOST Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (AA 121-180) antibody
SOST Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IF (p), ICC, IHC (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (AA 134-163) antibody
SOST Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB13011 unconjugated

anti-Sclerostin (SOST) (AA 38-66), (N-Term) antibody
SOST Reactivity: Human, Mouse WB Host: Rabbit Polyclonal RB36187 unconjugated

anti-Sclerostin (SOST) (N-Term) antibody
SOST Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (AA 25-209) antibody
SOST Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Sclerostin (SOST) (C-Term) antibody
Verified SOST Reactivity: Human IHC, ELISA Host: Goat Polyclonal unconjugated

Application Notes

WB: 1:1000. WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:500

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date

6 months
Zarei, Hulley, Sabokbar, Javaid: "Co-expression of DKK-1 and Sclerostin in Subchondral Bone of the Proximal Femoral Heads from Osteoarthritic Hips." in: Calcified tissue international, Vol. 100, Issue 6, pp. 609-618, (2018) (PubMed).

Lin, Lin, Chang, Wang, Lai: "Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation." in: Bioelectromagnetics, Vol. 36, Issue 7, pp. 494-505, (2015) (PubMed).
Target

Sclerostin (SOST)

Alternative Name

SOST (SOST Products)

Synonyms

LOC100313724 antibody, CDD antibody, VBCH antibody, 5430411E23Rik antibody, sclerostin antibody, LOC100313724 antibody, SOST antibody, Sost antibody

Background

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Molecular Weight

24031

Gene ID

50964

NCBI Accession

NP_079513

UniProt

Q9BQB4