CD47 Protein (CD47) (AA 19-139) (Fc Tag)
Quick Overview for CD47 Protein (CD47) (AA 19-139) (Fc Tag) (ABIN7274225)
Target
See all CD47 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 19-139
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Purification tag / Conjugate
- This CD47 protein is labelled with Fc Tag.
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Purpose
- Human CD47 Protein
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Sequence
- Gln19-Pro139
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Characteristics
- Recombinant Human CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Pro139.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD47 (CD47) (AA 19-139) protein (His tag)
CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (His tag)CD47 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (mFc-His Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD47 (CD47) (AA 19-139) protein (His-Avi Tag,Biotin)CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (His tag)CD47 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (Fc Tag)CD47 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag,AVI tag,Biotin)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) proteinCD47 Origin: Human Host: HEK-293 Cells Synthetic Nanodisc
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD47
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Alternative Name
- CD47
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Background
- CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
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Molecular Weight
- 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
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UniProt
- Q08722-1
Target
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