CD47 Protein (CD47) (AA 19-141) (His tag,mFc Tag)

Catalog No. ABIN6961081

This Recombinant CD47 protein is expressed in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (AA 19-141) (His tag,mFc Tag) (ABIN6961081)

Target: CD47

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Antibody Drug Assay (ADA), Screening Assay (ScA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-141

Purification tag / Conjugate: This CD47 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human CD47 protein with C-terminal mouse Fc and 6xHis tag

Specificity: CD47(Gln19-Glu141) CD47(Val198-Ala207) CD47(Ser257-Gly268) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: CD47,MER6,IAP,OA3
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Molecular Weight: predicted molecular mass of 60 kDa after removal of the signal peptide.

Gene ID: 961

UniProt: Q08722