CD47 Protein (CD47) (AA 19-140) (His tag)

Catalog No. ABIN7274237

Recombinant CD47 protein expressed in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (AA 19-140) (His tag) (ABIN7274237)

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-140

Purification tag / Conjugate: This CD47 protein is labelled with His tag.

Purpose: Mouse CD47 Protein

Sequence: Gln19-Lys140

Characteristics: Recombinant Mouse CD47 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Lys140.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).,Mouse CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Molecular Weight: 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.

UniProt: Q61735