CD47 Protein (CD47) (AA 19-141) (His tag)

Catalog No. ABIN7274217

This Recombinant CD47 protein is produced in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (AA 19-141) (His tag) (ABIN7274217)

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey, Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-141

Purification tag / Conjugate: This CD47 protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque CD47 Protein

Sequence: Gln19-Glu141

Characteristics: Recombinant Cynomolgus/Rhesus macaque CD47 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Glu141.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test).,Cynomolgus/Rhesus macaque CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus/Rhesus macaque CD47 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Molecular Weight: 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result.

UniProt: F7A802