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|Antigen||Ciliary Neurotrophic Factor (CNTF) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CNTF antibody is conjugated to Biotin Alternatives|
ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
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Product Details anti-CNTF AntibodyTarget Details CNTF Application Details Handling Images
E.coli derived recombinant Rat CNTF.
Type of Immunogen: Cell extract
Target Details CNTFProduct Details anti-CNTF Antibody Application Details Handling Images back to top
|Alternative Name||CNTF (CNTF Antibody Abstract)|
Name/Gene ID: CNTF
Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF
|Research Area||Stem Cells|
Application DetailsProduct Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
Approved: ELISA (1 μg/mL), IHC, Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat CNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat CNTF (LS-C104756) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CNTF Antibody Target Details CNTF Application Details Images back to top
|Reconstitution||Sterile PBS containing 0.1 % BSA|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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