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anti-Mouse (Murine) CSF2 antibody for Intracellular Staining

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Reactivity
Mouse (Murine)
371
118
44
27
14
10
4
4
2
2
Host
Rat
196
177
137
10
3
Clonality
Monoclonal
Conjugate
This CSF2 antibody is un-conjugated
59
16
13
13
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
340
278
124
80
75
69
60
53
35
24
20
18
12
7
6
6
6
4
3
3
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN1177288
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1002368 ICS IHC ELISA WB Rat IgG2a Log in to see

Top referenced anti-CSF2 antibody for Intracellular Staining

Similar anti-CSF2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies
ELISA 68 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Functional Studies (Func) 11 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 14 Antibodies

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Reactivity Mouse (Murine)
(371), (118), (44), (27), (14), (10), (4), (4), (2), (2)
Host Rat
(196), (177), (137), (10), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is un-conjugated
(59), (16), (13), (13), (7), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(340), (278), (124), (80), (75), (69), (60), (53), (35), (24), (20), (18), (12), (7), (6), (6), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN1177288) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse GM-CSF. Neutralization activity may be measured with a proliferation assay using 125 pg/mL recombinant mouse GM-CSF (Cat. No. 554586) to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse GM-CSF for 60 minutes).
50% Neutralization (ND50) at 0.2 - 2.1 myg/mL
> 90% Neutralization at 7 - 21 myg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Recombinant mouse GM-CSF
Clone MP1-22E9
Isotype IgG2a

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN1177288) Images back to top
Antigen
Alternative Name GM-CSF (CSF2 Antibody Abstract)
Background The MP1-22E9 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.
Research Area Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN1177288) Images back to top
Application Notes ELISA: Purified MP1-22E9 antibody (Cat. No. 554403 or 554404) can be useful as a capture antibody for sandwich ELISA for measuring mouse GM-CSF protein levels. Purified MP1-22E9 antibody can be paired with the biotinylated MP1-31G6 antibody (Cat. No. 554407) as the detection antibody, with recombinant mouse GM-CSF protein (Cat. No. 554586) as the standard. For testing mouse GM-CSF in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse GM-CSF ELISA Set (Cat. No. 555167).
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN1177288) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CSF2 Antibody (ABIN1177288)

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Product cited in:

Suda, OGarra, MacNeil, Fischer, Bond, Zlotnik: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, 1990

Nabel, Fresno, Chessman, Cantor: "Use of cloned populations of mouse lymphocytes to analyze cellular differentiation." in: Cell, Vol. 23, Issue 1, pp. 19-28, 1981