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Rat (Rattus) EPO ELISA Kit

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Antigen
Erythropoietin (EPO) ELISA Kits
  • EP
  • EPO
  • MVCD2
Epitope
AA 44-226
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
17
16
15
9
8
6
5
4
3
3
3
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
46.9-3000 pg/mL
Minimum Detection Limit
46.9 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2859223
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30647367 ABIN1114812 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN366461 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see 2
0.0012153484 ABIN579296 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see 3
0.0012153484 ABIN771753 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 10-250 mIU/mL 10 mIU/mL Log in to see
0.0012153484 ABIN1569611 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN955857 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN2748806 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012153484 ABIN2684143 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012153484 ABIN2748092 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see
0.0012153484 ABIN2533113 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN2081520 Sandwich ELISA Log in to see
0.0012153484 ABIN2947316 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN2112383 Competition ELISA Log in to see

Top referenced EPO ELISA Kit for ELISA

  • Rat (Rattus) EPO ELISA Kit for Sandwich ELISA - ABIN579296 (3 Publications): Costa, Fernandes, Ribeiro, Sereno, Garrido, Rocha-Pereira, Coimbra, Catarino, Belo, Bronze-da-Rocha, Vala, Alves, Reis, Santos-Silva: Aging is Associated with Impaired Renal Function, INF-gamma Induced Inflammation and with Alterations in Iron Regulatory Proteins Gene Expression. in Aging and disease 2014 (PubMed)

  • Rat (Rattus) EPO ELISA Kit for Sandwich ELISA - ABIN366461 (2 Publications): Guven Bagla, Ercan, Asgun, Ickin, Ercan, Yavuz, Bagla, Kaplan: Experimental acute myocardial infarction in rats: HIF-1α, caspase-3, erythropoietin and erythropoietin receptor expression and the cardioprotective effects of two different erythropoietin doses. in Acta histochemica 2013 (PubMed)

Similar EPO ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 14 ELISA Kits

General

Antigen Erythropoietin (EPO) ELISA Kits
Epitope AA 44-226
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(17), (16), (15), (9), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 46.9-3000 pg/mL
Minimum Detection Limit 46.9 pg/mL
Application ELISA
Supplier Log in to see

Product Details EPO ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of rat EPO in cell culture supernates, serum, plasma(heparin) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant rat EPO
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat EPO antibod - 1
  • Lyophilized recombinant rat EPO standar - 10ng/tubex2
  • Biotinylated anti- rat EPO antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: E.coli
Immunogen sequence: T44-K226

Target details

Product Details EPO ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name EPO (EPO ELISA Kit Abstract)
Background Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
Gene ID 24335
UniProt P29676
Research Area Cancer, Organogenesis, Hematopoietic Progenitors, Angiogenesis
Pathways JAK-STAT Signaling

Application Details

Product Details EPO ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol rat EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPO has been precoated onto 96-well plates. Standards(E.coli, T44-K226) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EPO amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat EPO standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 513, Standard deviation: 18.98, CV(%): 3.7
  • Sample 2: n=16, Mean(pg/ml): 1265, Standard deviation: 53.13, CV(%): 4.2
  • Sample 3: n=16, Mean(pg/ml): 1974, Standard deviation: 96.73, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 648, Standard deviation: 33.7, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 1450, Standard deviation: 82.65, CV(%): 5.7
  • Sample 3: n=24, Mean(pg/ml): 2301, Standard deviation: 145, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details EPO ELISA Kit Target details Application Details Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details EPO ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for EPO ELISA Kit (Erythropoietin) (ABIN2859223) Rat EPO PicoKine ELISA Kit standard curve