anti-Human IL-4 antibody for Immunohistochemistry (Frozen Sections)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Human
392
208
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rat
266
218
203
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
85
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
387
373
254
126
56
45
43
39
39
23
22
21
15
10
9
8
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN119373
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115763 EIA Func IHC (fro) Mouse IgG1 Log in to see 1
1 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2
1 ABIN217644 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
1 ABIN1859431 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 66-120 Log in to see Polyclonal
1 ABIN2475019 IHC (fro) FACS ELISA WB Rat IgG1 Log in to see MP4-25D2 4
1 ABIN601663 IHC (fro) FACS ELISA WB Rat IgG1 Log in to see
1 ABIN4325252 ELISA FACS IHC IHC (fro) WB DyLight 755 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325270 ELISA FACS IHC IHC (fro) WB Alexa Fluor 405 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325273 ELISA FACS IHC IHC (fro) WB Alexa Fluor 700 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325241 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0404-4Z33
1 ABIN4325243 ELISA FACS IHC IHC (fro) WB DyLight 350 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325245 ELISA FACS IHC IHC (fro) WB DyLight 405 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325248 ELISA FACS IHC IHC (fro) WB PerCP Rat IgG1 Log in to see MP4-25D2
1 ABIN4325254 ELISA FACS IHC IHC (fro) WB DyLight 488 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325271 ELISA FACS IHC IHC (fro) WB Alexa Fluor 488 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325272 ELISA FACS IHC IHC (fro) WB Alexa Fluor 647 Rat IgG1 Log in to see MP4-25D2
1 ABIN4325258 ELISA FACS IHC IHC (fro) WB DyLight 680 Rat IgG1 Log in to see MP4-25D2
1 ABIN576387 ELISA FACS IHC (fro) WB PE Rat IgG1 Log in to see
1 ABIN217540 ELISA FACS IHC (fro) WB Biotin Rat IgG1 Log in to see
1 ABIN3201264 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(392), (208), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rat
(266), (218), (203), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(85), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(387), (373), (254), (126), (56), (45), (43), (39), (39), (23), (22), (21), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images
Specificity This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.
Purification Affinity chromatography on Protein G
Immunogen Purified CHO cell expressed recombinant human IL-4.
Clone MP4-25D2
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 3565
NCBI Accession NP_000580
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-IL-4 antibody (ABIN119373)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012

Patterson, Behbahani, Kabat, Sullivan, OGorman, Landay, Flener, Khan, Yogev, Andersson: "Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 287-94, 2001

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998

Kubota, Miller, Imaoka, Kawabata, Fujihashi, McGhee, Kiyono: "Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5321-9, 1997

Carvalho, Bacellar, Brownell, Regis, Coffman, Reed: "Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5949-56, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) antibody (ABIN119373) Western blot analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN IN...