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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Blocking Antibody (Inhibition)||7 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||90 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||61 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Antigen||Interleukin 4 (IL4) Antibodies|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN119373) Images
|Specificity||This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Purified CHO cell expressed recombinant human IL-4.|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN119373) Images back to top
|Alternative Name||Interleukin-4 / IL4 (IL4 Antibody Abstract)|
|Background||IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN119373) Images back to top
Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN119373) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL-4 Antibody (ABIN119373)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Bie, Yuan, Zhao et al.: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012 (PubMed).
Patterson, Behbahani, Kabat et al.: "Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 287-94, 2001 (PubMed).
Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998 (PubMed).
Kubota, Miller, Imaoka et al.: "Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5321-9, 1997 (PubMed).
Carvalho, Bacellar, Brownell et al.: "Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5949-56, 1994 (PubMed).
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN119373) back to top