You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human IL-4 antibody for Blocking Antibody

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Human
406
215
50
25
22
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
268
225
220
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), Western Blotting (WB)
410
373
260
116
58
55
39
37
34
30
24
23
20
20
9
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967463
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN283749 Inhibition ELISA WB Mouse IgG2a Log in to see M6110641
0.0008696448 ABIN289347 Inhibition ELISA WB Rabbit Log in to see Polyclonal
0.0008696448 ABIN2689766 Inhibition ELISA Mouse IgG1, kappa Log in to see 8D4 2
0.0008696448 ABIN1002643 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008696448 ABIN636658 Inhibition ELISA WB Rabbit Log in to see Polyclonal
0.0008696448 ABIN2689767 Inhibition ELISA Mouse IgG1, kappa Log in to see 8D4

Top referenced anti-IL-4 antibody for Blocking Antibody

Similar anti-IL-4 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 212 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(406), (215), (50), (25), (22), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(268), (225), (220), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), Western Blotting (WB)
(410), (373), (260), (116), (58), (55), (39), (37), (34), (30), (24), (23), (20), (20), (9), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN967463) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Purified Recombinant Human IL-4
Clone MP4-25D2
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN967463) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested as a blocking control for intracellular staining.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN967463) Images back to top
Application Notes 1. Blocking Control for Intracellular Staining:
The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (ABIN967463) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

2. Neutralization:
The NA/LE™ MP4-25D2 antibody is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE ™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody.

3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN967463) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.

References for anti-IL-4 Antibody (ABIN967463)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Jung, Schauer, Rieger, Wagner, Einsle, Neumann, Heusser: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." in: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, 1995

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN967463) back to top
Supplier Images
 image for anti-IL-4 antibody (Interleukin 4) (ABIN967463) Expression of IL-4 by stimulated human peripheral blood mononuclear cells (PBMC). Hum...
 image for anti-IL-4 antibody (Interleukin 4) (ABIN967463) The binding of PE-MP4-25D2 antibody was blocked by preincubation of the fixed/permeab...