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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||76 Antibodies|
|Functional Studies (Func)||10 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||22 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||2 Antibodies|
|Antigen||Interleukin 4 (IL4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images
|Specificity||This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.|
|No Cross-Reactivity||Rat (Rattus)|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse.|
|Purification||Affinity Chromatography on protein G|
|Immunogen||Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
|Alternative Name||Interleukin-4 / IL4 (IL4 Antibody Abstract)|
|Background||Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN118404) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL-4 Antibody (ABIN118404)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Hochrein, OKeeffe, Luft et al.: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000 (PubMed).
Sander, Höidén, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) back to top