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anti-Mouse (Murine) IL-4 antibody for Immunohistochemistry (Frozen Sections)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Mouse (Murine)
329
181
50
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
244
199
152
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
90
47
31
23
18
8
7
6
4
4
4
2
1
1
1
1
Application
ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
341
303
186
84
56
31
27
27
24
23
20
20
19
18
10
6
5
5
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN118404
$ 566.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088039535 ABIN181862 EIA FACS IHC (fro) Rat IgG1 Log in to see BVD6-24G2 4
0.00088039535 ABIN217614 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
0.00088039535 ABIN255815 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see BVD4-1D11
0.00088039535 ABIN217637 FACS IHC (fro) ELISA Biotin Rat IgG1 Log in to see
0.00088039535 ABIN218103 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
0.00088039535 ABIN1862772 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-140 Log in to see Polyclonal
0.00088039535 ABIN601661 IHC (fro) FACS ELISA Rat IgG1 Log in to see
0.00088039535 ABIN2475024 IHC (fro) ELISA Biotin Rat IgG1 Log in to see BVD6-24G2 4
0.00088039535 ABIN217636 FACS IHC (fro) ELISA Rat IgG1 Log in to see
0.00088039535 ABIN2475022 IHC (fro) FACS ELISA WB Rat IgG2b Log in to see BVD4-1D11 4
0.00088039535 ABIN2475023 IHC (fro) FACS ELISA Rat IgG1 Log in to see BVD6-24G2 4

Top referenced anti-IL-4 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Mouse (Murine)
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Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 96 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 8 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 76 Antibodies
Functional Studies (Func) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(329), (181), (50), (25), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(244), (199), (152), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(90), (47), (31), (23), (18), (8), (7), (6), (4), (4), (4), (2), (1), (1), (1), (1)
Application ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(341), (303), (186), (84), (56), (31), (27), (27), (24), (23), (20), (20), (19), (18), (10), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images
Specificity This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on protein G
Immunogen Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Clone BVD4-1D11
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 16189
NCBI Accession NP_067258
UniProt P07750
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN118404) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL-4 Antibody (ABIN118404)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Hochrein, OKeeffe, Luft, Vandenabeele, Grumont, Maraskovsky, Shortman: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000

Sander, Höidén, Andersson, Möller, Abrams: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (ABIN118404) Western blot analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE IN...