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anti-Mouse (Murine) IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Mouse (Murine)
407
214
50
25
22
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
268
225
220
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
410
373
259
116
58
54
39
37
34
30
24
23
20
19
10
6
5
5
3
3
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN118404
$ 566.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN651434 FACS IHC (p) WB Rabbit Ig AA 122-150, C-Term Log in to see Polyclonal 1
0.13398378 ABIN3043168 WB Rabbit IgG AA 123-140, C-Term Log in to see Polyclonal
0.13398378 ABIN2704922 WB Rabbit C-Term Log in to see Polyclonal
0.13398378 ABIN255815 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see BVD4-1D11
0.13398378 ABIN2489868 WB Rat IgG1 Log in to see
0.13398378 ABIN1862772 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-140 Log in to see Polyclonal
0.13398378 ABIN2489309 IHC WB Rabbit Ig Fraction Log in to see Polyclonal
0.0011731827 ABIN115882 EIA Func WB Rabbit Log in to see Polyclonal 2
0.0011731827 ABIN115881 EIA Func WB Rabbit Log in to see Polyclonal 2
0.0011731827 ABIN264018 Func WB Rat IgG1 Log in to see I4-F5
0.0011731827 ABIN217614 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
0.0011731827 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
0.0011731827 ABIN115879 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011731827 ABIN115880 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011731827 ABIN1584604 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011731827 ABIN2624132 WB Rabbit IgG AA 123-140 Log in to see Polyclonal
0.0011731827 ABIN465884 ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0011731827 ABIN289356 Inhibition ELISA WB Rabbit Log in to see Polyclonal
0.0011731827 ABIN218103 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
0.0011731827 ABIN465839 Neut ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-IL-4 antibody for Western Blotting

Similar anti-IL-4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 129 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 8 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 109 Antibodies
Functional Studies (Func) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(407), (214), (50), (25), (22), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(268), (225), (220), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(410), (373), (259), (116), (58), (54), (39), (37), (34), (30), (24), (23), (20), (19), (10), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images
Specificity This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on protein G
Immunogen Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Clone BVD4-1D11
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 16189
NCBI Accession NP_067258
UniProt P07750
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN118404) Images back to top
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN118404) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL-4 Antibody (ABIN118404)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Hochrein, OKeeffe, Luft, Vandenabeele, Grumont, Maraskovsky, Shortman: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000

Sander, Höidén, Andersson, Möller, Abrams: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN118404) back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (ABIN118404) Western blot analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE IN...