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Mouse (Murine) IL12B ELISA Kit

Recommended IL12B Product (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) ELISA Kits
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Epitope
AA 23-335
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
31
11
9
7
5
5
4
4
3
3
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672845
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3087074 ABIN415600 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.3087074 ABIN2708536 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012127591 ABIN365448 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012127591 ABIN1884482 Sandwich ELISA 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012127591 ABIN2748835 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012127591 ABIN2042520 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012127591 ABIN1503292 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012127591 ABIN2684576 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012127591 ABIN1305259 Log in to see 7
0.0012127591 ABIN2535373 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012127591 ABIN455526 31.2-2000 pg/mL 31.2 pg/mL Log in to see

Top referenced IL12B ELISA Kit for ELISA

  • Mouse (Murine) IL12B ELISA Kit - ABIN1305259 (7 Publications): Chougnet, Freitag, Schito, Thomas, Sher, Shearer: In vivo CD40-CD154 (CD40 ligand) interaction induces integrated HIV expression by APC in an HIV-1-transgenic mouse model. in Journal of immunology (Baltimore, Md. : 1950) 2001 (PubMed)

  • Mouse (Murine) IL12B ELISA Kit for Sandwich ELISA - ABIN1672845 (5 Publications): Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. in Nature 2003 (PubMed)

Similar IL12B ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 12 ELISA Kits

General

Antigen Interleukin 12b (IL12B) ELISA Kits
Epitope AA 23-335
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(31), (11), (9), (7), (5), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 5 references available
Supplier Log in to see

Product Details IL12B ELISA Kit

Target details Application Details Handling References for IL12B Kit (ABIN1672845) Images
Purpose For quantitative detection of mouse IL-12(p40) in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL-12(p40)
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse IL-12(p40) antibod - 1
  • Lyophilized recombinant mouse IL-12(p40) standar - 10ng/tubex2
  • Biotinylated anti- mouse IL-12(p40) antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: M23-S335

Target details

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Antigen
Alternative Name IL12B (IL12B ELISA Kit Abstract)
Background Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40- kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35- kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.
Gene ID 16160
UniProt P43432
Research Area Hormones, Cytokines
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL-12(p40) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(p40) has been precoated onto 96-well plates. Standards(sf21, M23-S335) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p40) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(p40) amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(p40) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(p40) standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 136, Standard deviation: 8.3, CV(%): 6.1
  • Sample 2: n=16, Mean(pg/ml): 390, Standard deviation: 20.28, CV(%): 5.2
  • Sample 3: n=16, Mean(pg/ml): 683, Standard deviation: 31.42, CV(%): 4.6,
  • Sample 1: n=24, Mean(pg/ml): 165, Standard deviation: 11.55, CV(%): 7
  • Sample 2: n=24, Mean(pg/ml): 446, Standard deviation: 30.33, CV(%): 6.8
  • Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 40.1, CV(%): 5.6
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL12B Kit (ABIN1672845)

Product Details IL12B ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014

Background publications

Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain." in: Nature, Vol. 421, Issue 6924, pp. 744-8, 2003

Becher, Durell, Noelle: "Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12." in: The Journal of clinical investigation, Vol. 110, Issue 4, pp. 493-7, 2002

Wolf, Sieburth, Sypek: "Interleukin 12: a key modulator of immune function." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 2, pp. 154-68, 1994

Sieburth, Jabs, Warrington, Li, Lasota, LaForgia, Kelleher, Huebner, Wasmuth, Wolf: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992

Images

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Supplier Images
ELISA image for IL12B ELISA Kit (Interleukin 12b) (ABIN1672845) Mouse IL-12(p40) PicoKine ELISA Kit standard curve