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|Application / Reactivity||Mouse (Murine)|
|ELISA||12 ELISA Kits|
|Antigen||Interleukin 12b (IL12B) ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||15.6-1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|5 references available|
|Supplier||Log in to see|
Product Details IL12B ELISA KitTarget details Application Details Handling References for IL12B Kit (ABIN1672845) Images
|Purpose||For quantitative detection of mouse IL-12(p40) in cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)|
|Specificity||Natural and recombinant mouse IL-12(p40)|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: sf21
Immunogen sequence: M23-S335
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|Background||Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40- kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35- kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.|
|Research Area||Hormones, Cytokines|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||mouse IL-12(p40) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(p40) has been precoated onto 96-well plates. Standards(sf21, M23-S335) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p40) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(p40) amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(p40) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(p40) standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
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|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for IL12B Kit (ABIN1672845)Product Details IL12B ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Li, Li, Liu et al.: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014 (PubMed).
Cua, Sherlock, Chen et al.: "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain." in: Nature, Vol. 421, Issue 6924, pp. 744-8, 2003 (PubMed).
Becher, Durell, Noelle: "Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12." in: The Journal of clinical investigation, Vol. 110, Issue 4, pp. 493-7, 2002 (PubMed).
Wolf, Sieburth, Sypek: "Interleukin 12: a key modulator of immune function." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 2, pp. 154-68, 1994 (PubMed).
Sieburth, Jabs, Warrington et al.: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992 (PubMed).
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