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|Antigen||Interleukin 2 Receptor, beta (IL2RB) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL2 Receptor beta antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP)
|1 reference available|
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Product Details anti-IL2 Receptor beta AntibodyTarget Details IL2 Receptor beta Application Details Handling References for anti-IL2 Receptor beta antibody (ABIN120202) Images
|Specificity||This antibody recognises the CD122 cell surface glycoprotein. Clone TM-beta 1 is reported to block IL-2 high affinity binding to the IL-2R beta receptor. We recommend the use of SM2224LE for this purpose.|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Cells from murine IL-2RB+ transformant TART-mBeta. Lymph node (cells from immunised Sprague Dawley rats were fused with cells of the mouse PA1 myeloma cell line.|
Target Details IL2 Receptor betaProduct Details anti-IL2 Receptor beta Antibody Application Details Handling References for anti-IL2 Receptor beta antibody (ABIN120202) Images back to top
|Alternative Name||CD122 / IL2RB (IL2RB Antibody Abstract)|
|Background||CD122 is a 75-85kD molecule also known as interleukin-2 receptor (IL-2R) beta chain. In mouse, CD122 is primarily expressed on natural killer (NK) cells and at lower levels on lymphocyte subsets, monocytes and macrophages. CD122 can associate with the IL-2R alpha chain (CD25) and CD132 to form a functional high affinity receptor complex that binds IL-2. CD132/CD122 heterodimers can also be formed which bind IL-2 with intermediate affinity. IL-2 binding to the IL-2R is important in the generation and differentiation of T lymphocytes and NK cells.Synonyms: IL-2 receptor subunit beta, IL2 Receptor beta, Interleukin-2 receptor subunit beta, P70-75|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||JAK-STAT Signaling, Growth Factor Binding|
Application DetailsProduct Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Handling References for anti-IL2 Receptor beta antibody (ABIN120202) Images back to top
Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Application Details References for anti-IL2 Receptor beta antibody (ABIN120202) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL2 Receptor beta antibody (ABIN120202)Product Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Application Details Handling Images back to top
Tanaka, Tsudo, Karasuyama, Kitamura, Kono, Hatakeyama, Taniguchi, Miyasaka: "A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 7, pp. 2222-8, 1991