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anti-Human IL7 antibody for Immunoprecipitation

Recommended IL7 Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) Antibodies
  • A630026I06Rik
  • hlb368
  • Il-7
  • IL-7
  • IL7
Reactivity
Human
182
61
31
1
Host
Mouse
139
56
43
7
1
Clonality
Monoclonal
Conjugate
This IL7 antibody is un-conjugated
46
10
7
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
169
158
45
34
15
14
13
12
11
6
5
4
4
3
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN408959
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089419907 ABIN1825565 IP IHC ELISA WB Mouse IgG1 Log in to see NYRhIL7
0.00089419907 ABIN218154 IP IHC ELISA WB Mouse IgG1 Log in to see
0.00089419907 ABIN218149 IP ELISA WB Rat IgG2a, kappa Log in to see
0.00089419907 ABIN218148 IP Neut ELISA WB Rat IgG1, kappa Log in to see
0.00089419907 ABIN957623 IP ELISA WB Rat IgG1, kappa Log in to see BVD10-40F6
0.00089419907 ABIN957622 IP WB Rat IgG2a, kappa Log in to see BVD10-11C10
0.00089419907 ABIN1002664 ICS IP IHC ELISA WB Mouse IgG1 Log in to see NYRhIL7
0.00089419907 ABIN2144168 IP IHC ELISA WB Mouse IgG1 Log in to see NYRhIL7
0.00089419907 ABIN2289651 IP Neut ELISA WB Rat IgG1 kappa Log in to see 5K124
0.00089419907 ABIN2289653 IP ELISA WB Rat IgG2a kappa Log in to see 5K125
0.00089419907 ABIN2289670 IP IHC ELISA WB Mouse IgG1 Log in to see 5C66
0.00089419907 ABIN2289683 IP IHC ELISA WB Mouse IgG Log in to see 13F224
0.00089419907 ABIN2487830 IP IHC ELISA WB Mouse IgG1 Log in to see NYRhIL7-
0.00089419907 ABIN645733 ICC IP IHC ELISA WB Mouse IgG1 Log in to see
0.00089419907 ABIN808096 ICS IP IHC TCC ELISA Mouse Log in to see NYRhIL7

Similar anti-IL7 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 4 Antibodies
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 125 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Functional Studies (Func) 9 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Intracellular Flow Cytometry (ICFC) 5 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Neutralization (Neut) 29 Antibodies
Radioimmunoassay (RIA) 1 Antibodies

General

Antigen Interleukin 7 (IL7) Antibodies
Reactivity Human
(182), (61), (31), (1)
Host Mouse
(139), (56), (43), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL7 antibody is un-conjugated
(46), (10), (7), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(169), (158), (45), (34), (15), (14), (13), (12), (11), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-IL7 Antibody

Target Details IL7 Application Details Handling Images
Specificity Interleukin-7 (NYRhIL7)
Purification IgG purified
Immunogen r.Human IL-7
Clone NYRhIL7
Isotype IgG

Target Details IL7

Product Details anti-IL7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-7 / IL7 (IL7 Antibody Abstract)
Background Gene Symbol: IL7
Gene ID 3574
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
Application Notes By direct ELISA, 1:10,000 dilution will yield 0.3 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig
Recommended dilutions: ELISA 1:100-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Protocol Protocol specific for IL7 Antibody IHC-Paraffin and IHC-Frozen ProtocolDAY 1:I. Preparation of slides:1) Deparaffinization: soak tissue slides with xylene twice, each time for 15 minutes2) Rehydration: incubate slides in the following graded series of ethanol: 100 %I, 100 %II, 95 %, 90 %, 80 %, 70 %. 5 minutes for each solution. Then incubate in the water for 5 minutes.Note: The step 1 is only for paraffin embedded slide. For frozen slide, soak the slide in water for 5 minutes, and go to step 2.II. Protocol for Immunostaining:1) Immerse the slides in 0.3 % H2O2 (in distilled water) for 30 minutes at room temperature2) Rinse the slides with water followed by 1x PBS (pH7.
4) once, circle the tissue section with a Pap Pen (or Para-Pen, ImmEdge Pen)3) Incubate the slide with 1 % Normal serum/PBS [Mix 3.5 mL 1x PBS, pH 7.4 with 1 drops (about 35 µL/drop) of normal serum in a tube] for 30 minutes at room temperature4) Drop off the normal serum from the slides5) Incubate the sections with the PBS diluted first antibody (optimize the antibody titer before starting IHC) in a humid chamber for 1 hour to overnight at room temperature.DAY 2:6) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes7) Incubate the slides with PBS diluted Biotin-labeled secondary antibody for 30 minutes at room temperature. [Mix 1.4 mL 1xPBS pH 7.4 and 1 drop (about 35 µL/drop) of biotinylated anti-mouse IgG in a tube]8) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes9) Preparation of detection solution: Mix 1.33 mL 1xPBS, 1 drops (about 35 µL/drop) of Solution A, and 1 drops (about 35 µL/drop) of Solution B in a tube. Incubate the mixture at room temperature for 30 minutes before use.
) Add the detection solution prepared at step 9) to the tissue section. Incubate room temperature for 30 minutes.
) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes.
) Make fresh Development solution: Mix 1.6 mL DAB buffer, and 1 drop (about 35 µL/drop) of Liquid DAB in a tube.
) Add the Development solution to cover the tissues, and develop for 5-30 minutes14) Stop the reaction by soak the tissue in water.
) Counter stain the slides if necessary (Use Harris' Hematoxylin to stain the nuclear, and use eosin Y to stain cytosol)16) Dehydrate by soaked in the graded series of alcohol: 70 %-80 %-90 %-95 %-100 %I-100 %II, 3 minutes for each solution, then incubated in Xylene twice, 10 minutes each.
) Mounting the slides (Fisher's permount Cat. # SP15-100 or similar mounting material can be used)18) Add 2 drops (about 35 µL/drop) of detoxification solution into the used development solution. Incubate for 1 hour or overnight. Then it can be discarded.
Restrictions For Research Use only

Handling

Product Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Supplied in PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C