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Human IL7 ELISA Kit

Recommended IL7 Product (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) ELISA Kits
  • A630026I06Rik
  • hlb368
  • Il-7
  • IL-7
  • IL7
Epitope
AA 26-177
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
34
27
11
3
3
3
3
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN921098
$ 350.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32334948 ABIN924783 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.32334948 ABIN415146 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32334948 ABIN1113958 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32334948 ABIN2708294 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32334948 ABIN2114338 Cell Culture Supernatant, Serum, Plasma (EDTA) Log in to see
0.0012145225 ABIN578169 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.78-50 pg/mL 0.78 pg/mL Log in to see
0.0012145225 ABIN626488 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012145225 ABIN3198586 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012145225 ABIN625028 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 11
0.0012145225 ABIN2684727 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.5-50 pg/mL 1.5 pg/mL Log in to see 3
0.0012145225 ABIN1570751 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012145225 ABIN1446064 Cell Culture Supernatant, Serum, Plasma, Biological Samples 3 pg/mL Log in to see
0.0012145225 ABIN1112656 Sandwich ELISA Serum, Plasma, Cells 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2042561 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2114340 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012145225 ABIN2684728 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2684729 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN1446255 Cell Culture Supernatant, Plasma, Serum Log in to see
0.0012145225 ABIN1846973 Tissue Homogenate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN577099 Sandwich ELISA Log in to see

Top referenced IL7 ELISA Kit for ELISA

  • Human IL7 ELISA Kit for Sandwich ELISA - ABIN625028 (11 Publications): Meltzer, Standley: Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion. in The Journal of the American Osteopathic Association 2008 (PubMed)

  • Human IL7 ELISA Kit for Sandwich ELISA - ABIN921098 (3 Publications): Kröncke, Loppnow, Flad, Gerdes: Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. in European journal of immunology 1996 (PubMed)

Similar IL7 ELISA Kits

Application / Reactivity Human
ELISA 34 ELISA Kits

General

Antigen Interleukin 7 (IL7) ELISA Kits
Epitope AA 26-177
Reactivity Human
Kits with alternative reactivity to:
(34), (27), (11), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details IL7 ELISA Kit

Target details Application Details Handling References for IL7 Kit (ABIN921098) Images
Purpose For quantitative detection of human IL-7 in cell culture supernates, serum and plasma(EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-7
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- human IL-7 antibod - 1
  • Lyophilized recombinant human IL-7 standar - 10ng/tubex2
  • Biotinylated anti- human IL-7 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: D26-H177

Target details

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Antigen
Alternative Name IL7 (IL7 ELISA Kit Abstract)
Background IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.
Gene ID 3574
UniProt A8K673
Research Area Immunology, Innate Immunity, Cytokines, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, D26-H177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-7 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-7 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 54.5, Standard deviation: 3.38, CV(%): 6.2
  • Sample 2: n=16, Mean(pg/ml): 368, Standard deviation: 16.56, CV(%): 4.5
  • Sample 3: n=16, Mean(pg/ml): 759, Standard deviation: 36.67, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 55.9, Standard deviation: 3.86, CV(%): 6.9
  • Sample 2: n=24, Mean(pg/ml): 396, Standard deviation: 27.72, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 737, Standard deviation: 41.3, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details IL7 ELISA Kit Target details Application Details References for IL7 Kit (ABIN921098) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL7 Kit (ABIN921098)

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Background publications

Sawa, Arima, Ogura, Kitabayashi, Jiang, Fukushima, Kamimura, Hirano, Murakami: "Hepatic interleukin-7 expression regulates T cell responses." in: Immunity, Vol. 30, Issue 3, pp. 447-57, 2009

Kröncke, Loppnow, Flad, Gerdes: "Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction." in: European journal of immunology, Vol. 26, Issue 10, pp. 2541-4, 1996

Namen, Lupton, Hjerrild, Wignall, Mochizuki, Schmierer, Mosley, March, Urdal, Gillis: "Stimulation of B-cell progenitors by cloned murine interleukin-7." in: Nature, Vol. 333, Issue 6173, pp. 571-3, 1988

Images

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Supplier Images
ELISA image for IL7 ELISA Kit (Interleukin 7) (ABIN921098) Human IL-7 PicoKine ELISA Kit standard curve