Human IL7 ELISA Kit

Recommended IL7 Product (supplied by: Log in to see )

Antigen
Interleukin 7 (IL7) ELISA Kits
  • IL7
  • IL-7
  • A630026I06Rik
  • Il-7
  • hlb368
  • interleukin 7
  • interleukin 7-like
  • IL7
  • Il7
  • LOC100345409
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
37
26
14
6
3
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
5-1000 pg/mL
Minimum Detection Limit
5 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625028
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.70292 ABIN921098 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (EDTA) 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
9.70292 ABIN578169 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.78-50 pg/mL 0.78 pg/mL Log in to see
9.70292 ABIN415146 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
9.70292 ABIN3198586 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
9.70292 ABIN924783 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
9.70292 ABIN1113958 Sandwich ELISA Serum, Plasma, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Log in to see
9.70292 ABIN2708294 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.70292 ABIN5026943 Sandwich ELISA Plasma, Serum, Biological Fluids 7.8-500 pg/mL 7.8 pg/mL Log in to see
9.70292 ABIN2114338 Cell Culture Supernatant, Serum, Plasma (EDTA) Log in to see
1 ABIN1570751 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4993142 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.4-1000 pg/mL 1.4 pg/mL Log in to see
1 ABIN626488 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN2684727 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.5-50 pg/mL 1.5 pg/mL Log in to see 3
1 ABIN1446064 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 3 pg/mL Log in to see
1 ABIN5520858 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 7.813-500 pg/mL 7.813 pg/mL Log in to see
1 ABIN1112656 Sandwich ELISA Serum, Plasma, Cells 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2114340 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
1 ABIN2042561 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2684728 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2684729 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced IL7 ELISA Kit for ELISA

  • Human IL7 ELISA Kit for Sandwich ELISA - ABIN625028 (13 Publications): Meltzer, Standley: Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion. in The Journal of the American Osteopathic Association 2008 (PubMed)

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General

Antigen Interleukin 7 (IL7) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(37), (26), (14), (6), (3), (2), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 5-1000 pg/mL
Minimum Detection Limit 5 pg/mL
Application ELISA
Pubmed 13 references available
Supplier Log in to see

Product Details IL7 ELISA Kit

Target details Application Details Handling ProductDetails: References for IL7 Kit (ABIN625028) Images
Purpose Human IL-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity < 5 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

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Antigen
Alternative Name IL-7 (IL7 ELISA Kit Abstract)
Background IL-7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. It stimulates the proliferation of pre-B and pro-B-cells without affecting their differentiation and can replace murine bone marrow stromal cells in supporting the extended growth of both pre-B-cells and pro-B-cells. IL-7 may be of clinical significance for Adoptive immunotherapy since it is capable in vivo to cause the CD4 (+) T-cell-dependent destruction of tumor cells. The Human IL-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-7 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12.
Gene ID 3574
UniProt P13232
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details IL7 ELISA Kit Target details Handling ProductDetails: References for IL7 Kit (ABIN625028) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold*. *Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine, Assay Diluent B should be diluted 5-fold with deionized or distilled water into Item C vial to prepare 75 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 8 µL IL-7 standard from the vial of Item C, into a tube with 592 µL Assay Diluent A or 1x Assay Diluent B to prepare a 1,000 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200myl 200 µL 200 µL 200 µL 8 µL standard + 592 µL 1000 333.3 111.1 37.0 12.4 4.1 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 160-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 75 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a final 160 fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Human IL-7 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Human IL-7 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of IL-7 is typically less than 5 pg/mL.
Recovery: Recovery was determined by spiking various levels of human IL-7 into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 95.63 84-106 Plasma 96.37 85-108 Cell culture media 94.4 1 83-106
Linearity: Sample Type Serum Plasma Cell culture media 1:2 Average % of Expected 94 94 95 Range ( %) 83-104 84-105 86-105 1:4 Average % of Expected 94 93 93 Range ( %) 84-103 85-104 87-107
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IL7 ELISA Kit Target details Application Details ProductDetails: References for IL7 Kit (ABIN625028) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for IL7 Kit (ABIN625028)

Product Details IL7 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Patel, Okada, Hachey, Yang, Durum, Moreb, Chang: "Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation." in: BMC immunology, Vol. 13, pp. 46, 2012

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, 2011

Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, 2008

Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, 2008

Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, 2008

Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, 2008

Alderson, Sassenfeld, Widmer: "Interleukin 7 enhances cytolytic T lymphocyte generation and induces lymphokine-activated killer cells from human peripheral blood." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 577-87, 1990

Armitage, Namen, Sassenfeld, Grabstein: "Regulation of human T cell proliferation by IL-7." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 938-41, 1990

Alderson, Sassenfeld, Widmer: "Interleukin 7 enhances cytolytic T lymphocyte generation and induces lymphokine-activated killer cells from human peripheral blood." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 577-87, 1990

Armitage, Namen, Sassenfeld, Grabstein: "Regulation of human T cell proliferation by IL-7." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 938-41, 1990

Alderson, Sassenfeld, Widmer: "Interleukin 7 enhances cytolytic T lymphocyte generation and induces lymphokine-activated killer cells from human peripheral blood." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 577-87, 1990

Armitage, Namen, Sassenfeld, Grabstein: "Regulation of human T cell proliferation by IL-7." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 938-41, 1990

Background publications

Alderson, Sassenfeld, Widmer: "Interleukin 7 enhances cytolytic T lymphocyte generation and induces lymphokine-activated killer cells from human peripheral blood." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 577-87, 1990

Images

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Supplier Images
ELISA image for IL7 ELISA Kit (Interleukin 7) (ABIN625028) Interleukin 7 (IL7) ELISA Kit