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|Antigen||Leptin Receptor (LEPR) ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||156-10000 pg/mL|
|Minimum Detection Limit||156 pg/mL|
|4 references available|
|Supplier||Log in to see|
Product Details Leptin Receptor ELISA KitTarget details Application Details Handling References for Leptin Receptor Kit (ABIN1672740) Images
|Purpose||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Leptin receptor|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)|
Expression system for standard: NSO
Immunogen sequence: L22-G839
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: L22-G839
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|Alternative Name||LEPR (LEPR ELISA Kit Abstract)|
Protein Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
Background: Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
Synonyms: Leptin receptor,LEP-R,B219,OB receptor,OB-R,CD295,Lepr,Db, Obr,
Full Gene Name: Leptin receptorCellular Localisation: Cell membrane, Single-pass type I membrane protein.
|Pathways||JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
Sequence similarities: Belongs to the type I cytokine receptor family. Type 2 subfamily.
Tissue Specificity: Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.
|Protocol||mouse Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin receptor has been precoated onto 96-well plates. Standards(NSO, L22-G839) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin receptor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin receptor amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details Leptin Receptor ELISA Kit Target details Application Details References for Leptin Receptor Kit (ABIN1672740) Images back to top
|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for Leptin Receptor Kit (ABIN1672740)Product Details Leptin Receptor ELISA Kit Target details Application Details Handling Images back to top
Park, Shin, Park, Cheong, Cho, Lee, Lee, Lee, Oh, Kimm: "Polymorphisms in the leptin receptor (LEPR)--putative association with obesity and T2DM." in: Journal of human genetics, Vol. 51, Issue 2, pp. 85-91, 2006
Rosmond, Chagnon, Holm, Chagnon, Pérusse, Lindell, Carlsson, Bouchard, Björntorp: "Hypertension in obesity and the leptin receptor gene locus." in: The Journal of clinical endocrinology and metabolism, Vol. 85, Issue 9, pp. 3126-31, 2000
Clément, Vaisse, Lahlou, Cabrol, Pelloux, Cassuto, Gourmelen, Dina, Chambaz, Lacorte, Basdevant, Bougnères, Lebouc, Froguel, Guy-Grand: "A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction." in: Nature, Vol. 392, Issue 6674, pp. 398-401, 1998
Tartaglia, Dembski, Weng, Deng, Culpepper, Devos, Richards, Campfield, Clark, Deeds, Muir, Sanker, Moriarty, Moore, Smutko, Mays, Wool, Monroe, Tepper: "Identification and expression cloning of a leptin receptor, OB-R." in: Cell, Vol. 83, Issue 7, pp. 1263-71, 1996
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