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Mouse (Murine) Leptin Receptor ELISA Kit

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Antigen
Leptin Receptor (LEPR) ELISA Kits
  • cd295
  • CD295
  • db
  • diabetes
  • Fa
  • LEP-R
  • LEPR
  • Leprb
  • LEPRD
  • LEPROT
  • Modb1
  • OB-R
  • Ob-R
  • ob-rb
  • OB-RGRP
  • Ob-RM
  • obese-like
  • obl
  • obr
  • OBR
  • Obr
  • ObRb
  • Obrgrp
  • VPS55
Epitope
AA 22-839
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
15
10
4
3
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672740
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32684767 ABIN415516 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32684767 ABIN1117173 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32684767 ABIN2708467 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.32684767 ABIN2114468 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012099966 ABIN365462 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012099966 ABIN773380 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 25-500 pg/mL 25 pg/mL Log in to see
0.0012099966 ABIN1029100 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012099966 ABIN2042578 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012099966 ABIN2684857 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012099966 ABIN2536007 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012099966 ABIN455679 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012099966 ABIN625413 Sandwich ELISA Log in to see
0.0012099966 ABIN2089897 Sandwich ELISA Log in to see
0.0012099966 ABIN2116192 156-10000 pg/mL 156 pg/mL Log in to see
0.0012099966 ABIN2949093 31.25-2000 pg/mL 31.25 pg/mL Log in to see

Top referenced Leptin Receptor ELISA Kit for ELISA

Similar Leptin Receptor ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 16 ELISA Kits

General

Antigen Leptin Receptor (LEPR) ELISA Kits
Epitope AA 22-839
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (15), (10), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 4 references available
Supplier Log in to see

Product Details Leptin Receptor ELISA Kit

Target details Application Details Handling References for Leptin Receptor Kit (ABIN1672740) Images
Purpose For quantitative detection of mouse Leptin receptor in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse Leptin receptor
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- mouse Leptin receptor antibod - 1
  • Lyophilized recombinant mouse Leptin receptor standar - 10ng/tubex2
  • Biotinylated anti- mouse Leptin receptor antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L22-G839

Target details

Product Details Leptin Receptor ELISA Kit Application Details Handling References for Leptin Receptor Kit (ABIN1672740) Images back to top
Antigen
Alternative Name LEPR (LEPR ELISA Kit Abstract)
Background Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
Gene ID 16847
UniProt P48356
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details Leptin Receptor ELISA Kit Target details Handling References for Leptin Receptor Kit (ABIN1672740) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin receptor has been precoated onto 96-well plates. Standards(NSO, L22-G839) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin receptor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin receptor amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1328, Standard deviation: 87.65, CV(%): 6.6
  • Sample 2: n=16, Mean(pg/ml): 3836, Standard deviation: 168.8, CV(%): 4.4
  • Sample 3: n=16, Mean(pg/ml): 6429, Standard deviation: 327.9, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 1656, Standard deviation: 125.9, CV(%): 7.6
  • Sample 2: n=24, Mean(pg/ml): 4025, Standard deviation: 221.4, CV(%): 5.5
  • Sample 3: n=24, Mean(pg/ml): 6872, Standard deviation: 474.2, CV(%): 6.9
Restrictions For Research Use only

Handling

Product Details Leptin Receptor ELISA Kit Target details Application Details References for Leptin Receptor Kit (ABIN1672740) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Leptin Receptor Kit (ABIN1672740)

Product Details Leptin Receptor ELISA Kit Target details Application Details Handling Images back to top
Background publications

Park, Shin, Park, Cheong, Cho, Lee, Lee, Lee, Oh, Kimm: "Polymorphisms in the leptin receptor (LEPR)--putative association with obesity and T2DM." in: Journal of human genetics, Vol. 51, Issue 2, pp. 85-91, 2006

Rosmond, Chagnon, Holm, Chagnon, Pérusse, Lindell, Carlsson, Bouchard, Björntorp: "Hypertension in obesity and the leptin receptor gene locus." in: The Journal of clinical endocrinology and metabolism, Vol. 85, Issue 9, pp. 3126-31, 2000

Clément, Vaisse, Lahlou, Cabrol, Pelloux, Cassuto, Gourmelen, Dina, Chambaz, Lacorte, Basdevant, Bougnères, Lebouc, Froguel, Guy-Grand: "A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction." in: Nature, Vol. 392, Issue 6674, pp. 398-401, 1998

Tartaglia, Dembski, Weng, Deng, Culpepper, Devos, Richards, Campfield, Clark, Deeds, Muir, Sanker, Moriarty, Moore, Smutko, Mays, Wool, Monroe, Tepper: "Identification and expression cloning of a leptin receptor, OB-R." in: Cell, Vol. 83, Issue 7, pp. 1263-71, 1996

Images

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Supplier Images
ELISA image for Leptin Receptor ELISA Kit (LEPR) (ABIN1672740) Mouse Leptin receptor PicoKine ELISA Kit standard curve