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Mouse (Murine) Prolactin ELISA Kit

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Antigen
Prolactin (PRL) ELISA Kits
  • AV290867
  • prl
  • PRL
  • Prl1a1
  • PRLB
  • PRLSD1
  • Prol
  • prolactin
  • RATPRLSD1
  • RNPROL
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
28
21
17
11
10
8
4
4
3
3
3
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
0.82-200 ng/mL
Minimum Detection Limit
0.82 ng/mL
Application
ELISA
Supplier
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Catalog No. ABIN2506964
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN415572 Sandwich ELISA Serum, Plasma, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see 1
0.30628014 ABIN1672761 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) 156-10000 pg/mL 156 pg/mL Log in to see 3
0.30628014 ABIN1116667 Sandwich ELISA Serum, Plasma, Biological Fluids 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.30628014 ABIN1029638 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.30628014 ABIN2010774 Sandwich ELISA 0.1-100 ng/mL 0.1 ng/mL Log in to see
0.30628014 ABIN2708591 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.0012155735 ABIN365131 Competition ELISA Serum, Plasma, Tissue Homogenate 0.8-28 ng/mL 0.8 ng/mL Log in to see 1
0.0012155735 ABIN3032836 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 27.4-20000 pg/mL 27.4 pg/mL Log in to see
0.0012155735 ABIN625422 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 30-20000 pg/mL 30 pg/mL Log in to see 5
0.0012155735 ABIN512358 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 10-250 pg/mL 10 pg/mL Log in to see
0.0012155735 ABIN456250 Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155735 ABIN2042641 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012155735 ABIN1326881 Sandwich ELISA Log in to see 2
0.0012155735 ABIN2685286 Sandwich ELISA Plasma (heparin), Plasma (citrate), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012155735 ABIN956299 Log in to see
0.0012155735 ABIN2685287 Sandwich ELISA Plasma 3-200 ng/mL 3 ng/mL Log in to see
0.0012155735 ABIN2538214 Sandwich ELISA Serum, Plasma, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.0012155735 ABIN2102564 Sandwich ELISA Log in to see
0.0012155735 ABIN1995293 Sandwich ELISA Serum, Plasma Log in to see
0.0012155735 ABIN2950668 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced Prolactin ELISA Kit for ELISA

Similar Prolactin ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 22 ELISA Kits

General

Antigen Prolactin (PRL) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(28), (21), (17), (11), (10), (8), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 0.82-200 ng/mL
Minimum Detection Limit 0.82 ng/mL
Application ELISA
Supplier Log in to see

Product Details Prolactin ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Prolactin in mouse serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of mouse Prolactin.
Sensitivity 80 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • mouse Prolactin Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1 x8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details Prolactin ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Prolactin (PRL ELISA Kit Abstract)
Background Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. Originally characterized as a lactogenic hormone, further studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. Mouse Prolactin cDNA encodes a 228 amino acid (aa) residue protein with a putative 31 aa residue signal peptide.
Molecular Weight 22.6 kDa
Gene ID 19109
NCBI Accession NP_001157002
UniProt P06879
Research Area Signaling, Hormones
Pathways JAK-STAT Signaling

Application Details

Product Details Prolactin ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 2.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse Prolactin has been pre-coated onto a microplate. Standards, samples and mouse Prolactin mix are pipetted into the wells and any Prolactin present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 500 ng/mL standard. Gently vortex to mix.Take 200 μL mouse Prolactin standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 200 ng/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. mouse Prolactin Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:5.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Mouse Prolactin Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 2 hours at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details Prolactin ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details Prolactin ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Prolactin ELISA Kit (PRL) (ABIN2506964) Prolactin (PRL) ELISA Kit