Mouse (Murine) Prolactin ELISA Kit

Recommended Prolactin Product (supplied by: Log in to see )

Antigen
Prolactin (PRL) ELISA Kits
  • PRL
  • prolactin
  • PRLB
  • PRLSD1
  • Prl1a1
  • Prol
  • RATPRLSD1
  • RNPROL
  • AV290867
  • prl
  • prolactin
  • prolactin, gene 3
  • PROLACTIN protein
  • prolactin-like
  • PRL
  • prl.3
  • Prl
  • LOC100136792
  • LOC100136580
  • PROLACTIN
  • LOC101843376
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
39
27
23
16
16
13
5
5
4
3
3
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
30-20000 pg/mL
Minimum Detection Limit
30 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN625422
$ 666.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN2506964 Sandwich ELISA Serum, Plasma 0.82-200 ng/mL 0.82 ng/mL Log in to see
10.090268 ABIN1029638 Sandwich ELISA Biological Fluids, Plasma, Serum 0.156-10 ng/mL 0.156 ng/mL Log in to see
10.090268 ABIN365131 Competition ELISA Serum, Plasma, Tissue Homogenate 0.8-28 ng/mL 0.8 ng/mL Log in to see 1
10.090268 ABIN415572 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see 1
10.090268 ABIN1672761 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) 156-10000 pg/mL 156 pg/mL Log in to see 3
10.090268 ABIN1116667 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
10.090268 ABIN2708591 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
10.090268 ABIN2010774 Sandwich ELISA 0.1-100 ng/mL 0.1 ng/mL Log in to see
1 ABIN3032836 Competition ELISA Cell Culture Supernatant, Plasma, Serum 27.4-20000 pg/mL 27.4 pg/mL Log in to see
1 ABIN512358 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 10-250 pg/mL 10 pg/mL Log in to see
1 ABIN5592562 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5658103 Sandwich ELISA Biological Fluids, Plasma, Serum 0.625 ng/mL - 10 ng/mL 0.625 ng/mL Log in to see
1 ABIN5658104 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 27.4 pg/mL - 20000 pg/mL 27.4 pg/mL Log in to see
1 ABIN456250 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5521489 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2042641 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
1 ABIN2685286 Sandwich ELISA Plasma (heparin), Plasma (citrate), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN1326881 Sandwich ELISA Log in to see 2
1 ABIN5068255 Sandwich ELISA Plasma, Serum Log in to see
1 ABIN2685287 Sandwich ELISA Plasma 3-200 ng/mL 3 ng/mL Log in to see

General

Antigen Prolactin (PRL) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(39), (27), (23), (16), (16), (13), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 30-20000 pg/mL
Minimum Detection Limit 30 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Prolactin ELISA Kit

Target details Application Details Handling References for Prolactin Kit (ABIN625422) Images
Purpose Mouse Prolactin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity 30 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Prolactin ELISA Kit Application Details Handling References for Prolactin Kit (ABIN625422) Images back to top
Antigen
Alternative Name Prolactin (PRL ELISA Kit Abstract)
Background Prolactin (PRL)
UniProt P06879
Research Area Signaling, Hormones
Pathways JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus

Application Details

Product Details Prolactin ELISA Kit Target details Handling References for Prolactin Kit (ABIN625422) Images back to top
Application Notes Recommended Dilution for serum and plasma samples5 - 100 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. 2. Sample dilution: Assay Diluent A (Item D) is used for dilution of serum/plasma samples, Assay Diluent C (Item L) can be used for dilution of cell culture supernates. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µl Assay Diluent A (for serum/plasma samples) or Assay Diluent C (for cell culture supernates) into Item C vial to prepare a 50 ng/ml standard. Dissolve the powder thoroughly by a gentle mix. Add 180 µl Prolactin standard (50 ng/ml) from the vial of Item C, into a tube with 270 µl Assay Diluent A or Assay Diluent C to prepare a 20,000 pg/ml standard solution. Pipette 400 µl Assay Diluent A or Assay Diluent C into each tube. Use the 20,000 pg/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Gently vortex to mix. Assay Diluent A or Assay Diluent C serves as the zero standard (0 pg/ml). 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µl of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 10,000-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 2 µl of HRP-Streptavidin concentrate into a tube with 198 µl 1x Assay Diluent B to prepare a 100-fold diluted HRP- Streptavidin solution (do not store the diluted solution for next day use). Mix through and then pipette 100 µl of prepared 100-fold diluted solution into a tube with 10 ml 1x Assay Diluent B to prepare a final 10,000 fold diluted HRP-Streptavidin solution.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µl of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µl of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µl of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µl of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µl of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Restrictions For Research Use only

Handling

Product Details Prolactin ELISA Kit Target details Application Details References for Prolactin Kit (ABIN625422) Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for Prolactin Kit (ABIN625422)

Product Details Prolactin ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Hesling, Lopez, Fattet, Gonzalo, Treilleux, Blanchard, Losson, Goffin, Pigat, Puisieux, Mikaelian, Gillet, Rimokh: "Tif1γ is essential for the terminal differentiation of mammary alveolar epithelial cells and for lactation through SMAD4 inhibition." in: Development (Cambridge, England), Vol. 140, Issue 1, pp. 167-75, 2012

Chen, Stairs, Boxer, Belka, Horseman, Alvarez, Chodosh: "Autocrine prolactin induced by the Pten-Akt pathway is required for lactation initiation and provides a direct link between the Akt and Stat5 pathways." in: Genes & development, Vol. 26, Issue 19, pp. 2154-68, 2012

Hesling, Lopez, Fattet, Gonzalo, Treilleux, Blanchard, Losson, Goffin, Pigat, Puisieux, Mikaelian, Gillet, Rimokh: "Tif1? is essential for the terminal differentiation of mammary alveolar epithelial cells and for lactation through SMAD4 inhibition." in: Development (Cambridge, England), Vol. 140, Issue 1, pp. 167-75, 2012

Images

Product Details Prolactin ELISA Kit Target details Application Details Handling References for Prolactin Kit (ABIN625422) back to top
Supplier Images
ELISA image for Prolactin (PRL) ELISA Kit (ABIN625422) Prolactin (PRL) ELISA Kit