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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||3 Antibodies||25 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||8 Antibodies||23 Antibodies|
|ELISA (Detection)||5 Antibodies|
|Antigen||Thrombopoietin (THPO) Antibodies|
|Epitope||AA 22-259 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Thrombopoietin antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Thrombopoietin AntibodyTarget Details Thrombopoietin Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: thrombopoietin
Protein Name: Thrombopoietin
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.|
Target Details ThrombopoietinProduct Details anti-Thrombopoietin Antibody Application Details Handling Images back to top
|Alternative Name||Thpo (THPO Antibody Abstract)|
Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Synonyms: C mpl ligand | MGDF | MKCSF | ML | MPL ligand | MPLLG | THCYT1 | THPO | Thrombopoietin | TPO | P40225
|Pathways||JAK-STAT Signaling, Hormone Activity|
Application DetailsProduct Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.