anti-Rat (Rattus) Thrombopoietin antibody for ELISA

Recommended Thrombopoietin Antibody (supplied by: Log in to see )

Antigen
Thrombopoietin (THPO) Antibodies
  • tpo
  • LOC100231762
  • TPO
  • MGDF
  • MKCSF
  • ML
  • MPLLG
  • THCYT1
  • Mgdf
  • Ml
  • Mpllg
  • Tpo
  • Tpo1
  • Tpo2
  • Tpo3
  • Tpo4
  • thrombopoietin
  • thrombopoietin-like
  • THPO
  • thpo
  • LOC100231762
  • Thpo
  • LOC100620258
  • LOC100357079
  • LOC100855822
Epitope
AA 22-259
6
6
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
150
43
10
4
2
1
1
1
1
1
1
Host
Rabbit
88
59
30
16
Clonality
Polyclonal
Conjugate
This Thrombopoietin antibody is un-conjugated
13
8
5
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
111
21
20
11
10
10
7
6
5
3
3
2
2
2
1
1
1
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Catalog No. ABIN5518876
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2574344 ELISA WB Rabbit IgG AA 24-266 Log in to see Polyclonal
1 ABIN2902553 ELISA IF/ICC IHC WB Rabbit AA 24-266 Log in to see Polyclonal

General

Antigen Thrombopoietin (THPO) Antibodies
Epitope AA 22-259
(6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(150), (43), (10), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(88), (59), (30), (16)
Clonality
Polyclonal
Conjugate This Thrombopoietin antibody is un-conjugated
(13), (8), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (111), (21), (20), (11), (10), (10), (7), (6), (5), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-Thrombopoietin Antibody

Target Details Thrombopoietin Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: thrombopoietin
Protein Name: Thrombopoietin
Purification Immunogen affinity purified.
Immunogen E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.
Isotype IgG

Target Details Thrombopoietin

Product Details anti-Thrombopoietin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Thpo (THPO Antibody Abstract)
Background Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

Synonyms: C mpl ligand | MGDF | MKCSF | ML | MPL ligand | MPLLG | THCYT1 | THPO | Thrombopoietin | TPO | P40225
Gene ID 21832
UniProt P40226
Pathways JAK-STAT Signaling, Hormone Activity

Application Details

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.