CRP ELISA Kit

Catalog No. ABIN1672879

Rat CRP ELISA Kit, Colorimetric assay for quantification of Rat CRP, that has been mentioned in 9+ publications.

Quick Overview for CRP ELISA Kit (ABIN1672879)

Target: CRP (C-Reactive Protein (CRP))

Binding Specificity: AA 20-230

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details CRP ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Rat CRP/C Reactive Protein ELISA Kit to quantitate Rat Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: H20-S230

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.9%, 5.4%, 6.4%
Inter-Assay Precision (CV%): 5%, 7.2%, 7.4%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for CRP ELISA Kit (ABIN1672879)

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Zhao, Han: "Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy." in: Medical science monitor basic research, Vol. 24, pp. 84-92, (2018) (PubMed).

Zou, Huang, Lu, Fang, Wang, Zhao, Jia, Xu, Wang, Wang, Dong: "The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 165, (2017) (PubMed).

Li, Su, Xu, Huang, Xu: "Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides." in: Neuropsychiatric disease and treatment, Vol. 13, pp. 2477-2487, (2017) (PubMed).

Wang, Chai, He, Ai, Sun, Huang, Li: "Intermittent hypoxia simulating obstructive sleep apnea causes pulmonary inflammation and activates the Nrf2/HO-1 pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3463-3470, (2017) (PubMed).

Lu, Li, Li, Wang, Wu, Li, Wang: "Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1359105, (2016) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Shi, Song, Zhang, Li, Li: "Correlation between the microinflammatory state and left ventricular structural and functional changes in maintenance haemodialysis patients." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 532-536, (2013) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." in: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive Protein

Background:

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Gene Full Name: C-reactive protein

Gene ID: 25419

UniProt: P48199

Pathways: Carbohydrate Homeostasis