(1 reference)-
- Target See all Cathepsin B (CTSB) Kits
- Cathepsin B (CTSB)
- Detection Method
- Fluorometric
- Application
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
- Top Product
- Discover our top product CTSB ELISA Kit
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Cathepsin B (CTSB) ELISA Kit
CTSB Reactivity: Human Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Mouse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
SensoLyte® 440 Cathepsin B Assay KitFRET Fluorometric
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Mouse AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Human AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cathepsin B (CTSB) ELISA KitCTSB Reactivity: Mouse Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- Comment
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FRET-based Assay Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect Components A and B from light and moisture.
- Storage
- -80 °C
- Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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- Target
- Cathepsin B (CTSB)
- Alternative Name
- Cathepsin B (CTSB Products)
- Synonyms
- APPS Kit, CPSB Kit, CB Kit, apps Kit, cpsb Kit, LOC692390 Kit, CTSB Kit, cathepsin B Kit, cathepsin B S homeolog Kit, CTSB Kit, Ctsb Kit, ctsb.S Kit, LOC692390 Kit
- Background
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
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