DNMT3A ELISA Kit

Catalog No. ABIN2043357

Product Details DNMT3A ELISA Kit

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: DNA Methyltransferase 3A (DNMT3A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 3A (DNMT3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 3A (DNMT3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 3A (DNMT3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DNA Methyltransferase 3A (DNMT3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNA Methyltransferase 3A (DNMT3A) (DNMT3A Products)

Synonyms: DNMT3A ELISA Kit, DNMT3A2 ELISA Kit, M.HsaIIIA ELISA Kit, MmuIIIA ELISA Kit, dnmt3a1 ELISA Kit, dnmt6 ELISA Kit, DNA methyltransferase 3 alpha ELISA Kit, DNA methyltransferase 3A ELISA Kit, DNA (cytosine-5-)-methyltransferase 3 alpha b ELISA Kit, DNMT3A ELISA Kit, Dnmt3a ELISA Kit, dnmt3a ELISA Kit, dnmt3ab ELISA Kit