HSPB3 ELISA Kit

Catalog No. ABIN2043619

Product Details HSPB3 ELISA Kit

Target: HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.29 ng/mL

Application Details

Plate: Pre-coated

Protocol: Heat Shock Protein Beta 3 (HSPb3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 3 (HSPb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 3 (HSPb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 3 (HSPb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein Beta 3 (HSPb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HSPB3

Target: HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))

Alternative Name: Heat Shock Protein beta 3 (HSPb3) (HSPB3 Products)

Synonyms: HSPB3 ELISA Kit, DHMN2C ELISA Kit, HMN2C ELISA Kit, HSPL27 ELISA Kit, 2310035K17Rik ELISA Kit, AI844863 ELISA Kit, Hsbp3 ELISA Kit, spb3 ELISA Kit, heat shock protein family B (small) member 3 ELISA Kit, heat shock 27kDa protein 3 ELISA Kit, heat shock protein 3 ELISA Kit, HSPB3 ELISA Kit, hspb3 ELISA Kit, Hspb3 ELISA Kit