PIP ELISA Kit

Catalog No. ABIN2044285

Product Details PIP ELISA Kit

Target: PIP (Prolactin-Induced Protein (PIP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.87-3000 pg/mL

Minimum Detection Limit: 46.87 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 16.23 pg/mL

Application Details

Plate: Pre-coated

Protocol: Prolactin Induced Protein (PIP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein (PIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin Induced Protein (PIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin Induced Protein (PIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prolactin Induced Protein (PIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: Prolactin Induced Protein (PIP) (PIP Products)

Synonyms: GCDFP-15 ELISA Kit, GCDFP15 ELISA Kit, GPIP4 ELISA Kit, GP17 ELISA Kit, mSMGP ELISA Kit, prolactin induced protein ELISA Kit, PIP ELISA Kit, Pip ELISA Kit