PKC alpha ELISA Kit
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- Target See all PKC alpha (PKCa) ELISA Kits
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.054 ng/mL
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- Discover our best selling PKCa ELISA Kit
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- Discover our top product PKCa ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Protein Kinase C Alpha (PKCa) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Alpha (PKCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Alpha (PKCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Alpha (PKCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase C Alpha (PKCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all PKC alpha (PKCa) ELISA Kits
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- Alternative Name
- Protein Kinase C alpha (PKCa) (PKCa Products)
- Synonyms
- pkc-alpha ELISA Kit, pkcalpha ELISA Kit, im:7139045 ELISA Kit, si:dkey-8l19.1 ELISA Kit, LOC100219195 ELISA Kit, AAG6 ELISA Kit, PKC-alpha ELISA Kit, PKCA ELISA Kit, PRKACA ELISA Kit, AI875142 ELISA Kit, Pkca ELISA Kit, PKRCA ELISA Kit, protein kinase C alpha ELISA Kit, protein kinase C, alpha L homeolog ELISA Kit, protein kinase C, alpha ELISA Kit, PRKCA ELISA Kit, prkca.L ELISA Kit, prkca ELISA Kit, Prkca ELISA Kit
- Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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