FITC Annexin V Apoptosis Detection Kit with 7-AAD

Details for Product No. ABIN2669212, Supplier: Log in to see
Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
Alternatives
Human Annexin V ELISA Kit
Reactivity
Mammalian
17
15
15
14
13
8
7
7
4
2
2
2
2
1
1
1
1
Conjugate
FITC
Application
Multiplex Assay (MA)
Options
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Characteristics FITC Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our 7-amino-actinomycin D (7-AAD) solution. Early apoptotic cells will exclude 7-AAD, while late stage apoptotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Components FITC Annexin V Apoptosis Detection Kit with 7-AAD
Alternative Name Annexin V (ANXA5 ELISA Kit Abstract)
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Precaution of Use Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with 7-AAD (ABIN2669212) FITC Annexin V Apoptosis Detection Kit with 7-AAD
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with 7-AAD (ABIN2669212) FITC Annexin V Apoptosis Detection Kit with 7-AAD (Image 2)
Background publications Maciel, Neves, Santinha, Reis, Domingues, Teresa Cruz, Pitt, Spickett, Domingues: "Detection of phosphatidylserine with a modified polar head group in human keratinocytes exposed to the radical generator AAPH." in: Archives of biochemistry and biophysics, Vol. 548, pp. 38-45, 2014 (PubMed).