Endothelin 1 FLISA Kit
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- Target See all Endothelin 1 (EDN1) FLISA Kits
- Endothelin 1 (EDN1)
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Reactivity
- Human, Mouse, Rat, Pig, Dog, Cow
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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- Discover our top product EDN1 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Endothelin 1 (EDN1) FLISA Kits
- Endothelin 1 (EDN1)
- Alternative Name
- Endothelin-1 (ET-1) (EDN1 Products)
- Synonyms
- et1 FLISA Kit, et-1 FLISA Kit, Edn1 FLISA Kit, EDN FLISA Kit, ET-1 FLISA Kit, ET1 FLISA Kit, PPET1 FLISA Kit, HDLCQ7 FLISA Kit, preproET FLISA Kit, Et1 FLISA Kit, fb14d01 FLISA Kit, wu:fb14d01 FLISA Kit, PPET-1 FLISA Kit, EDN1 FLISA Kit, edn1 FLISA Kit, edn1-a FLISA Kit, edn1-b FLISA Kit, endothelin 1 FLISA Kit, endothelin 1 S homeolog FLISA Kit, endothelin 1 L homeolog FLISA Kit, edn1 FLISA Kit, EDN1 FLISA Kit, Edn1 FLISA Kit, edn1.S FLISA Kit, edn1.L FLISA Kit
- Pathways
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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