Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor) (HGF) ELISA Kit

Antigen: Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor) (HGF)

Synonyms: SF, HGFB, HPTA, F-TCF, DFNB39, NK1, NK2, HGF/SF, SF/HGF, C230052L06Rik, HGF, CACNA2D1

Reactivity: Human

Application: ELISA

Catalog no.: ABIN411278

Additional Information

Characteristics: For quantitative detection of human HGF in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: Hepatocyte Growth Factor (HGF)

Description: Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.5 The standard product used in this kit is recombinant human HGF with the molecular mass of 60-70KDa.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 5pg/ml. Range: 31.2pg/ml-2000pg/ml

Application Details

Principle: This human HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human HGF specific-specific monoclonal antibodies (clone No. 24516) were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HGF amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant human HGF standard: 10ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

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Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor) (HGF) ELISA Kit