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Dynactin 1 ELISA Kit

DCTN1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882617
  • Target See all Dynactin 1 (DCTN1) ELISA Kits
    Dynactin 1 (DCTN1)
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Dynactin subunit 1/DCTN1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human Peroxiredoxin 4.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Dynactin 1 (DCTN1) ELISA Kits
    Dynactin 1 (DCTN1)
    Alternative Name
    Dynactin Subunit 1 (DCTN1 Products)
    Synonyms
    DAP-150 ELISA Kit, DP-150 ELISA Kit, P135 ELISA Kit, Chip150 ELISA Kit, DCTN1 ELISA Kit, DKFZp469C1813 ELISA Kit, AL022633 ELISA Kit, Glued ELISA Kit, p150 ELISA Kit, p150 ELISA Kit, CG9206 ELISA Kit, DYNA_DROME ELISA Kit, Dmel\\CG9206 ELISA Kit, FCP-D ELISA Kit, Glu ELISA Kit, P150 ELISA Kit, P150[Glued] ELISA Kit, p150/Glued ELISA Kit, p150/glued ELISA Kit, p150[Glued] ELISA Kit, p150[glued] ELISA Kit, t1 ELISA Kit, Dynactin-1 ELISA Kit, glued ELISA Kit, dynactin subunit 1 ELISA Kit, dynactin 1 ELISA Kit, Dynactin 1, p150 subunit ELISA Kit, dynactin subunit 1 L homeolog ELISA Kit, DCTN1 ELISA Kit, Dctn1 ELISA Kit, DCTN1-p150 ELISA Kit, dctn1.L ELISA Kit
    Gene ID
    1639
    UniProt
    Q14203
    Pathways
    M Phase, ER-Nucleus Signaling
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