SULT2A1 ELISA Kit

Catalog No. ABIN4884692

Product Details SULT2A1 ELISA Kit

Target: SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human SULT2A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SULT2A1

Target: SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))

Alternative Name: SULT2A1 (SULT2A1 Products)

Synonyms: dhea-st ELISA Kit, dheas ELISA Kit, st2 ELISA Kit, st2a1 ELISA Kit, st2a3 ELISA Kit, sta ELISA Kit, std ELISA Kit, SULT2A1 ELISA Kit, ST2A1 ELISA Kit, Std ELISA Kit, Sth1 ELISA Kit, mSTa1 ELISA Kit, STa ELISA Kit, Smp-2 ELISA Kit, St2 ELISA Kit, St2a1 ELISA Kit, Sth2 ELISA Kit, DHEA-ST ELISA Kit, DHEAS ELISA Kit, HST ELISA Kit, ST2 ELISA Kit, ST2A3 ELISA Kit, STD ELISA Kit, hSTa ELISA Kit, sulfotransferase family 2A member 1 ELISA Kit, sulfotransferase family 2A member 1 L homeolog ELISA Kit, alcohol sulfotransferase ELISA Kit, sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1 ELISA Kit, SULT2A1 ELISA Kit, sult2a1.L ELISA Kit, PCC8801_2155 ELISA Kit, Cyan8802_2217 ELISA Kit, NAL212_1203 ELISA Kit, sult2a1 ELISA Kit, Sult2a1 ELISA Kit

Gene ID: 6822

UniProt: Q06520

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process