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CCL3 ELISA Kit

CCL3 Reactivity: Human Colorimetric Sandwich ELISA 23.44-1500 pg/mL Plasma, Serum
Catalog No. ABIN5026947
  • Target See all CCL3 ELISA Kits
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    Reactivity
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    23.44-1500 pg/mL
    Minimum Detection Limit
    23.44 pg/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the in vitro quantitative determination of Human MIP-1Alpha /CCL3 concentrations in serum, plasma and other biological fluids.
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensitivity
    < 14 pg/mL
    Components
    plate, standard, diluent, antibodies
    Material not included
    pipettes, tubes, reader, incubator
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    Discover our top product CCL3 ELISA Kit
  • Sample Volume
    100 μL
    Assay Time
    3 - 4 h
    Plate
    Pre-coated
    Protocol
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1Alpha /CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1Alpha /CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1Alpha /CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MIP-1Alpha /CCL3 can be calculated using the standard curve.
    Restrictions
    For Research Use only
  • Precaution of Use
    protect your eyes
    Storage
    4 °C
  • Target See all CCL3 ELISA Kits
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    Alternative Name
    MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha) (CCL3 Products)
    Synonyms
    MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Autophagy
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