Annexin A2 ELISA Kit

Catalog No. ABIN5651833

Product Details Annexin A2 ELISA Kit

Target: Annexin A2 (ANXA2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A2 (ANXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A2 (ANXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A2 (ANXA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: Annexin A2 (ANXA2 Products)

Synonyms: ANX2 ELISA Kit, ANX2L4 ELISA Kit, CAL1H ELISA Kit, LIP2 ELISA Kit, LPC2 ELISA Kit, LPC2D ELISA Kit, P36 ELISA Kit, PAP-IV ELISA Kit, AW215814 ELISA Kit, Cal1h ELISA Kit, p36 ELISA Kit, anx2 ELISA Kit, lip2 ELISA Kit, lpc2 ELISA Kit, cal1h ELISA Kit, lpc2d ELISA Kit, pap-i ELISA Kit, anx2l4 ELISA Kit, MGC76145 ELISA Kit, ANXA2 ELISA Kit, anxa2 ELISA Kit, anxa2-a ELISA Kit, anxa2-b ELISA Kit, annexin A2 ELISA Kit, annexin A2 S homeolog ELISA Kit, ANXA2 ELISA Kit, Anxa2 ELISA Kit, anxa2 ELISA Kit, anxa2.S ELISA Kit

Background:

Gene Name: Annexin A2

Gene Aliases: ANX-A2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, Protein I, Chromobindin-8, Calpactin-1 heavy chain, Lipocortin II, Placental anticoagulant protein IV

Gene ID: 302

UniProt: P07355

Pathways: S100 Proteins