CA1 ELISA Kit

Catalog No. ABIN5652550

Product Details CA1 ELISA Kit

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 pg/mL - 100 pg/mL

Minimum Detection Limit: 1.56 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase I (CA1). No significant cross-reactivity or interference between Carbonic Anhydrase I (CA1) and analogues was observed.

Sensitivity: 0.65 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase I (CA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase I (CA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase I (CA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase I (CA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase I (CA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase I (CA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: Carbonic Anhydrase I (CA1 Products)

Synonyms: CA-I ELISA Kit, CAB ELISA Kit, Car1 ELISA Kit, AW555628 ELISA Kit, Ca1 ELISA Kit, Car-1 ELISA Kit, BG:DS00941.1 ELISA Kit, CAH ELISA Kit, CG7820 ELISA Kit, Dmel\\CG7820 ELISA Kit, Gh7 ELISA Kit, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 ELISA Kit, ATBCA1 ELISA Kit, ATSABP3 ELISA Kit, BETA CARBONIC ANHYDRASE 1 ELISA Kit, F4P13.5 ELISA Kit, F4P13_5 ELISA Kit, SABP3 ELISA Kit, SALICYLIC ACID-BINDING PROTEIN 3 ELISA Kit, carbonic anhydrase 1 ELISA Kit, CA1 ELISA Kit, LOC100135826 ELISA Kit, GB15888 ELISA Kit, carbonic anhydrase 1 ELISA Kit, Carbonic anhydrase 1 ELISA Kit, carbonic anhydrase I ELISA Kit, carbonic anhydrase ELISA Kit, CA1 ELISA Kit, Car1 ELISA Kit, CAH1 ELISA Kit, LOC100135826 ELISA Kit, LOC408827 ELISA Kit, Tsp_05114 ELISA Kit, LOC100153915 ELISA Kit, LOC100050192 ELISA Kit, Ca1 ELISA Kit

Background:

Gene Name: Carbonic Anhydrase I

Gene Aliases: CA-I, CAI, Car1, CAB, Carbonic anhydrase B, Carbonate dehydratase I

Gene ID: 759

UniProt: P00915