LY75/DEC-205 ELISA Kit

Catalog No. ABIN5656520

Product Details LY75/DEC-205 ELISA Kit

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 75 (LY75). No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: Lymphocyte Antigen 75 (LY75 Products)

Synonyms: LY75 ELISA Kit, MGC140308 ELISA Kit, DEC205 ELISA Kit, im:7150926 ELISA Kit, si:dkey-78p20.2 ELISA Kit, LYT75 ELISA Kit, CD205 ELISA Kit, DEC-205 ELISA Kit, CLEC13B ELISA Kit, GP200-MR6 ELISA Kit, LY-75 ELISA Kit, RGD1565962 ELISA Kit, Ly-75 ELISA Kit, lymphocyte antigen 75 ELISA Kit, CD205 protein ELISA Kit, CD302 antigen ELISA Kit, LY75 ELISA Kit, Cyan7822_2917 ELISA Kit, LY5 ELISA Kit, ly75 ELISA Kit, cd205 ELISA Kit, LOC100380704 ELISA Kit, LOC100433861 ELISA Kit, LOC100483541 ELISA Kit, Ly75 ELISA Kit

Background:

Gene Name: Lymphocyte Antigen 75

Gene Aliases: CD205, LY75, CLEC13B, DEC-205, GP200-MR6, C-type lectin domain family 13 member B

Gene ID: 17076

UniProt: Q60767