MYL9 ELISA Kit

Catalog No. ABIN5657106

Product Details MYL9 ELISA Kit

Target: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 9, Regulatory (MYL9). No significant cross-reactivity or interference between Myosin Light Chain 9, Regulatory (MYL9) and analogues was observed.

Sensitivity: 27 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 9, Regulatory (MYL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 9, Regulatory (MYL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 9, Regulatory (MYL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 9, Regulatory (MYL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 9, Regulatory (MYL9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 9, Regulatory (MYL9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYL9

Target: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Alternative Name: Myosin Light Chain 9, Regulatory (MYL9 Products)

Synonyms: LC20 ELISA Kit, MLC-2C ELISA Kit, MLC2 ELISA Kit, MRLC1 ELISA Kit, MYRL2 ELISA Kit, AI327049 ELISA Kit, MLC20 ELISA Kit, Mylc2c ELISA Kit, RLC-C ELISA Kit, myosin ELISA Kit, regulatory ELISA Kit, MRCL3 ELISA Kit, MRLC2 ELISA Kit, MYL9 ELISA Kit, zgc:103467 ELISA Kit, myl9 ELISA Kit, zgc:77916 ELISA Kit, myosin light chain 9 ELISA Kit, myosin, light polypeptide 9, regulatory ELISA Kit, myosin light chain 9 L homeolog ELISA Kit, myosin, light chain 9, regulatory ELISA Kit, myosin, light chain 12A, regulatory, non-sarcomeric ELISA Kit, myosin, light chain 9a, regulatory ELISA Kit, myosin, light chain 9b, regulatory ELISA Kit, MYL9 ELISA Kit, Myl9 ELISA Kit, myl9.L ELISA Kit, MYL12A ELISA Kit, myl9a ELISA Kit, myl9b ELISA Kit

Background:

Gene Name: Myosin Light Chain 9, Regulatory

Gene Aliases: MLC2, LC20, MYRL2, MRLC1, 20 kDa myosin light chain, Myosin Regulatory Light Chain 2,Smooth Muscle Isoform, Myosin Regulatory Light Chain 1

Gene ID: 10398

UniProt: P24844