NPEPPS ELISA Kit

Catalog No. ABIN5658306

Product Details NPEPPS ELISA Kit

Target: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Puromycin Sensitive Aminopeptidase (PSA). No significant cross-reactivity or interference between Puromycin Sensitive Aminopeptidase (PSA) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Puromycin Sensitive Aminopeptidase (PSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Puromycin Sensitive Aminopeptidase (PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Puromycin Sensitive Aminopeptidase (PSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Puromycin Sensitive Aminopeptidase (PSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Puromycin Sensitive Aminopeptidase (PSA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Puromycin Sensitive Aminopeptidase (PSA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NPEPPS

Target: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Alternative Name: Puromycin Sensitive Aminopeptidase (NPEPPS Products)

Synonyms: APS ELISA Kit, KLK2A1 ELISA Kit, PSA ELISA Kit, hK3 ELISA Kit, AAP-S ELISA Kit, MP100 ELISA Kit, CG1009 ELISA Kit, Dmel\\CG1009 ELISA Kit, dPSA ELISA Kit, dPsa ELISA Kit, l(3)06226 ELISA Kit, GB13772 ELISA Kit, Psa ELISA Kit, R74825 ELISA Kit, goku ELISA Kit, psa ELISA Kit, fb68d07 ELISA Kit, sb:cb848 ELISA Kit, wu:fb68d07 ELISA Kit, NPEPPS ELISA Kit, npepps ELISA Kit, MGC147526 ELISA Kit, kallikrein related peptidase 3 ELISA Kit, aminopeptidase puromycin sensitive ELISA Kit, Puromycin sensitive aminopeptidase ELISA Kit, puromycin-sensitive aminopeptidase ELISA Kit, aminopeptidase puromycin sensitive L homeolog ELISA Kit, KLK3 ELISA Kit, NPEPPS ELISA Kit, Psa ELISA Kit, LOC410769 ELISA Kit, Npepps ELISA Kit, npepps ELISA Kit, npepps.L ELISA Kit

Background:

Gene Name: Puromycin Sensitive Aminopeptidase

Gene Aliases: NPEPPS, AAP-S, Metalloproteinase MP100, Cytosol alanyl aminopeptidase

Gene ID: 9520

UniProt: P55786